内脂素对ii型糖尿病模型小鼠病理发展的长期影响-中国试验动物学报.doc

周传农修改： 英文摘要 — 已修改 正文中的英文书写 — 已顺 图的英文 — 已顺 文献的英文书写 — 已顺 Tg-visfatin×ob/ob小鼠表型特征与内脂素表达 关菲菲，全雄志，张连峰* [摘要] 目的 内脂素（visfatin ）是近年发现的一种在内脏脂肪的因子，研究表明胰岛素抵抗。 [关键词]： 内脂素; 转基因; ob/ob小鼠; 2型糖尿病; 胰岛素耐受 [中图分类号] [文献标识码] [文章编号] Phenotypic characteristics and visfatin expression in Tg-visfatin×ob/ob mice GUAN Fei-fei, QUAN Xiong-zhi, GAO Shan, ZHANG Xiao-juan, GE Wen-ping, ZHANG Lian-feng* (Institute of Laboratory Animal Sciences, Peking Union Medical College Chinese Academy of Medical Sciences, Key Laboratory of Human Disease Comparative Medicine, Minstry of Health, Beijing 100021, China) [Abstract] Objective Visfatin is an adipokine associated with insulin resistance and type 2 diabetes (T2D). However, its effects are controversial in published reports. The aim of the present study was to determine the phenotypic characteristics of Tg-visfatin×ob/ob mice and explore the effect of visfatin on Tg-visfatin mice and type 2 diabete mice ob/ob. Methods Tg-visfatin mice were crossed with ob(+/?)mice to produce homozygote Tg-visfatin×ob/ob mice and ob/ob mice. The body weight of the two types of mice were recorded from 1 to 9 months of age. Intraperitoneal glucose tolerance test (IPGTT) and insulin tolerance test (ITT) were made at 3-, 5-, and 9-month-old mice, and the death rate of the mice were recorded from 9 to 11 month of age. Results The body weight from 1 to 9 months of age was almost the same. From the results of IPGTT and ITT, compared with ob/ob mice, at 3 months of age, the glucose tolerance and insulin tolerance of Tg-visfatin×ob/ob mice was reduced; at 5 months of age, only glucose tolerance of Tg-visfatin×ob/ob mice was reduced; when at 9 months of age, the glucose tolerance and insulin tolerance of Tg-visfatin×ob/ob mice was aggravated. The death rate of Tg-visfatin×ob/ob mice was improved by 44.4% than that of ob/ob mice from 9 to 11 months of age. Conclusions The results demonstrate that visfatin plays a positive role in attenuating glucose tolerance and insulin toler