仪器分析（instrumental analysis）.doc

仪器分析（instrumental analysis） The second chapter gas chromatography analysis 1. when the following parameters change: (1) the column length is shortened; (2) the stationary phase changes; (3) the flow velocity of the mobile phase increases; (4) does the decrease in the partition coefficient result from the decrease in comparison; why? A: the change in the stationary phase causes a change in the partition coefficient because the partition coefficient is only related to the nature of the component and the nature of the stationary phase and the mobile phase Therefore, (1) shortening of column length will not result in change of distribution coefficient (2) the change of stationary phase results in the change of partition coefficient (3) increasing the velocity of the mobile phase will not cause the change of the distribution coefficient (4) a decrease in the distribution coefficient does not result in a decrease in comparison 2. when the following parameters change: (1) the column length increases; (2) the stationary phase volume increases; (3) the flow velocity decreases; (4) does it cause the change of the distribution ratio when compared with the increase; why? Answer: k=K/b, and b=VM/VS, the distribution ratio, in addition to the composition, the nature of the two-phase, column temperature, column pressure outside, and also compared with, and with the flow phase flow velocity, column length independent Reason: (1) not change, (2) increase, (3) do not change, (4) decrease 3. can the possibility of separation be judged according to the number of theoretical plates? Why? Answer: No, the effective plate number only indicates the column efficiency and the column separation capability, while the possibility of separation depends on the difference of the partition coefficient between the stationary phase and the mobile phase 4. in a 2 m long column, a mixture of analysis, get the following data: the retention time of benzene, toluene, ethylbenzene and were 1 20 , 2 and 2 3