20(S)-人参皂苷Rh2 对人结直肠癌Caco-2 与HT-29 细胞增殖影响.doc

20(S)-人参皂苷Rh2 对人结直肠癌Caco-2 和HT-29 细胞增殖的影响 李秋影1, 2，颜璐璐2，张莉华2，张兰兰2，马晓慧2，郭治昕2 * （1. 天津中医药大学，天津，300193；2. 天津天士力研究院药理毒理所，天津，300410） 摘 要：目的 本实验探讨20(S)-人参皂苷Rh2 对人结直肠癌Caco-2 和HT-29 细胞增殖及凋亡的影响。方法 采用荧光微孔法测定20(S)-人参皂苷Rh2 对体外培养的人结直肠癌Caco-2、HT-29 细胞增殖的影响，并计算抑制率；利用显微镜观察给药后细胞形态变化。结果 20(S)-人参皂苷Rh2 对Caco-2、HT-29 细胞生长有抑制作用，并呈剂量依赖性；20(S)-人参皂苷Rh2 作用48h 后HT-29、 Caco-2 细胞达到半数抑制浓度（IC50）为20.66、27.18ug·mL-1；显微镜显示HT-29 细胞经25ug/ml 20(S)-人参皂苷Rh2 作用后细胞之间互相脱离，成多边形状，细胞破碎。 结论 20(S)-人参皂苷Rh2 可以抑制Caco-2、HT-29 细胞生长并导致其凋亡。 关键词：20(S)-人参皂苷Rh2；HT-29；Caco-2；细胞凋亡 Effect of 20(S)-ginsenoside Rh2 on the proliferation of human colon cancer cells Caco-2 and HT-29 LI Qiu-ying1, YAN Lu-lu2, ZHANG Li-hua2, ZHANG Lan-lan2, Ma Xiao-hui2, GUO Zhi-xin2 * (1.Tianjin University of Traditional Chinese Medicine, Tianjin 300193,China; 2：The Department of Pharmaclogy and Toxicology of Tasly，Tianjin，300410） Abstract: OBJECTIVE To investigate the effects of 20(s)-ginsenoside-Rh2 on the proliferation of human colon cancer cell lines Caco-2 and HT-29。METHODS Cellgrowth inhibition was evaluated by Cell based Fluorescent Staining Digital Image High Content Screening assay. Cellular morphology was observed under Microscopy. RESULTS 20(s)-ginsenoside Rh2 inhibited the proliferation of Caco-2、HT-29 cells in a dose-dependent manner. The IC50 of 20.66、27.18ug·mL-1 were detected after Caco-2、HT-29 cells treated with 20 (s)-ginsenoside-Rh2 for 48 hour; Microscopy showed obviously apoptosis morphologic changes of HT-29 and Caco-2 cells were treated with different 20(s)-ginsenoside-Rh2. CONCLUSION 20(s)-ginsenoside Rh2 exhibits the growth inhibition effects and induces apoptosis in the test concentration on human colon cancer Caco-2 and HT-29 cells. Key words: 20(S)-ginsenoside Rh2; HT-29; Caco-2; Apoptosis 人参为五加科人参属植物人参（Panax ginseng C.A. meyer）的干燥根，是已有近几千年应用历史的名贵中药。现代医学研究表明，人参包含有机酸、维生素、糖类、无机盐、固醇、寡肽、多糖、挥发油类和人参皂苷等多种成分，具有抗肿瘤、抗衰老、抗辐射等多种生物活性[1-4]。人参皂苷作为人参中一类主要药效成分，在癌症的预防和治疗方面具有较强的活性，其中以人参皂苷R-Rg3 为主要成分的抗癌新药参—胶囊已作为中药一类新药上市[5]。 大量研究报道，人参皂苷-Rh2