hiv感染者特异抗原及核酸测定（Detection of specific antigen and nucleic acid in HIV infected persons）.docVIP

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hiv感染者特异抗原及核酸测定（Detection of specific antigen and nucleic acid in HIV infected persons）.doc

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hiv感染者特异抗原及核酸测定（Detection of specific antigen and nucleic acid in HIV infected persons）

hiv感染者特异抗原及核酸测定（Detection of specific antigen and nucleic acid in HIV infected persons） Detection of specific antigen and nucleic acid in HIV infected persons 2009-12-13 Wu Nanping, Liu Kezhou, Chen Zhi, Li inherited R.H.Dennin In order to explore the relationship between HIV, DNA, RNA, P24Ag and host and the internal relationship between the three. Methods 139 cases of anti HIV positive blood samples were collected, and P24Ag (U.S.Abbort) was measured by solid phase immunoassay, and HIV, RNA (U.S.Roche, Bra) and nested polymerase chain reaction (HIV, DNA) (Marke, Germany) were measured by reverse transcriptase polymerase chain reaction (PCR). The sensitivity and consistency of three kinds of HIV detection methods for the same specimen were studied. Materials and methods Sample source From October 1995 to October 1996, a total of 139 patients with anti -HIV positive (ELISA) infection were collected from the clinical medical center of Lubeck Medical University in Germany. The plasma levels of P24, HIV, DNA, HIV and RNA were measured. Two, HIV, P24 antigen determination P24 antigen by solid-phase double antibody sandwich ELISA Kit (USA Abbort) detection, P24 monoclonal antibody (mouse) package test serum 40 C is on a solid support and a series of diluted lines, after washing with anti HIV-Ag (Rabbit) 40 DEG C for 1 hours, after washing with anti IgG enzyme free combination compound (Coat), 40 DEG C for 1 hours after washing with OPD color, color depth according to the measured A, the sensitivity can reach PG level, the process has the specificity test. Three, HIV, RNA extraction Quantitative determination of HIV and RNA reagents was provided by the Roche Branchburg company of the United states. 200 L EDTA plasma was extracted by, purified by isopropanol, washed with 70% alcohol, and extracted at RNA. HIV quantitative standard strain (QS) provided by the company of synthetic RNA containing specific probe, given a certain amount of RNA amplification with the primers use