大鼠闭胸体外循环模型的建立.pdfVIP

复 里学握(医学版) Fudan Univ J Med Sei 大鼠闭胸体外循环模型的建立 陈 纲 贾 兵 何春兰 闫宪刚 叶 明 陶麒麟 (复旦大学附属儿科医院心脏外科 上海 200032) 【摘要】 目的 建立Sprague Dawley(SD)大鼠体外循环模型，为体外循环脑、肺、肝、肾等重要脏器损伤的基础 研究提供经济实用的动物模型。方法 采用右颈内动脉插管灌注、右颈外静脉插管至右房引流方法进行 SD大 鼠闭胸体外循环转流，低温诱发室颤心脏停跳。将24只大鼠随机平均分为体外循环 120 min组、深低温低流量 60 min组、深低温停循环60 min组，术中进行有创血压、心电图监测和动脉、混合静脉血气分析。结果 24只大 鼠均顺利建立体外循环模型，血气指标均正常范围内，摆脱体外循环时平均血压维持 50 mmHg以上，停体外循 环后心肺功能均顺利恢复。结论 本实验建立大鼠闭胸不同体外循环模型是切实可行的，该模型是研究体外循 环生理的一个可信赖的平台，有利于研究体外循环后多脏器损伤的病理生理变化。 【关键词】 动物模型； 体外循环； 大鼠 【中图分类号】 R 645．1 【文献标识码】 A The establishment of the animal model of closed chest cardiopulmonary bypass in Sprague Dawley rats CHEN Gang~，JIA Bing，HE Chun—lan，YAN Xian—gang，YE Ming，TAO Qi—lin (Department of Cardiac Surgery，Children’s Hospital，Fudan University，Shanghai 200032，China) [Abstract] Objective To establish an animal model of closed chest cardiopulmonary bypass(CPB)in Sprague-Dawley(SD)rats． Methods The SD rats were assigned tO undergo CPB via right carotid and jugular cannulation，and selbdesigned CPB circuit．The cardiac arrest was induced by hypothermal ventricular fibrillation．Twenty-four rats were equally divided into three groups which underwent CPB for 1 20 minutes，deep hypothermic lOW flow(DH LF)bypass 60 minutes，deep hypothermic circulatory arrest(DHCA)60 minutes individually．During bypass，invasive arterlal blood pressure，electrocardio— graph and arterial and mixed venous blood gas analysis were monitored． Results The CPB was per— formed successfully in all the rats．The blood gas analysis results were all maintained in normal range and the mean arterial pressure was over 50 mmHg when off-pump．The cardiac and respiratory func— tions recovered after the rat weaned from the CPB． Conclusions The animal model of closed chest CP