不同浓度人参皂苷RG3对高转移卵巢癌细胞集落形成及细胞形态影响.docVIP

不同浓度人参皂苷RG3对高转移卵巢癌细胞集落形成及细胞形态影响摘 要:目的:观察人参皂苷(Rg3)对高转移人卵巢癌细胞系H0-8910PM的细胞集落形成和细胞形态的影响。方法:采用本院自建的高转移人卵巢癌细胞系H0-8910PM进行实验,随机分成5组:①空白对照组:只加新鲜的全培养液;②低浓度Rg3组:10μg/mL Rg3培养液;③中浓度Rg3组:20μg/mL Rg3培养液;④中高浓度Rg3组: 30μg/mL Rg3培养液;⑤高浓度Rg3组:40μg/mL Rg3培养液。观察不同浓度Rg3分别对细胞集落形成率和细胞形态的影响。结果:经单因素分析发现5组细胞随着Rg3浓度的升高,细胞集落形成率逐渐而下降,差异有统计学意义(F=176.362,P=0.000)。多组变量随机区组分析示:空白对照组与任何浓度Rg3组比较差异均有统计学意义(P=0.000,0.000,0.000,0.000)。低浓度组与中高浓度Rg3两组细胞集落形成率结果相近(8.3% vs 6.9%),差异无统计学意义(P=0.133)。中高浓度组与高浓度Rg3两组细胞集落形成率均很低(2.5% Vs 0.78%),差异无统计学意义(P=0.057)。低浓度组、中高浓度组分别与中高浓度组、高浓度组比较,差异均有统计学意义(P=0.000,0.000,0.000,0.000)。中浓度以上Rg3可引起H0-8910PM的细胞形态结构损伤。中高浓度及高浓度Rg3在微观结构上能造成更严重的细胞形态结构损伤。结论: Rg3对高转移人卵巢癌H0-8910PM细胞能有效减少细胞集落的形成。中高、高浓度的Rg3(30μg/mL以上)尤其可以产生较好的抑制肿瘤细胞生长作用,并呈一定的剂量依赖性,且对细胞形态结构破坏上产生更重的损伤。 关键词:人高转移; 卵巢肿瘤; HO-8910PM细胞系;人参皂苷Rg3;集落形成率;细胞形态 中图分类号:R737.31 文献标识码:A 文章编号:1673-7717(2010)01-0118-04 Influence of ginsenoside Rg3 on cell colony-forming efficiency and morphocytology of human high-metastasis ovarian cancer cell line H0-8910PM in vitro CHEN Lu?1, QIAN Li?juan?2, GU Lin?hui?2, MA Sheng?lin?2, ZHU Jian?qing?1 (1.Department of Gynecology Oncology, Zhejiang Cancer Hospital, Hangzhou 310022 Zhejiang, China;2. Zhejiang Cancer Research Institute, Hangzhou310022 Zhejiang, China) Abstract:Objective:To observe the effect of cell colony-forming efficiency and morphocytology with Ginsenoside Rg3 on high-metastasis human ovarian cancer cell line H0-8910PM.Methods: The cell line were divided into 5 groups randomly: ①blank control group: only adding fresh complete culture medium; ②low consistency Rg3 group: 10μg/mL Rg3 medium; ③middle consistency Rg3 group: 20μg/mL Rg3 medium; ④middle-high consistency Rg3 group: 30μg/mL Rg3 medium; ⑤high consistency Rg3 group: 40μg/mL Rg3 medium. In this study cell colony-forming efficiency and morphocytology changement were used to investigate the effects of cell proliferation suppression. Results: Univariance analysis showed in these 5 groups the higher concentration of Rg