二氢青蒿素抑制兔耳瘢痕增生实验探究.doc

二氢青蒿素抑制兔耳瘢痕增生实验探究[摘要]目的：观察二氢青蒿素对兔耳增生性瘢痕增生的影响。方法：选取成年新西兰大耳白兔16只，建立兔耳增生性瘢痕动物模型，待上皮化后，对实验组、对照组分别给予二氢青蒿素（180mg/kg）和生理盐水（乙醇终浓度为0.1%）各10ml灌胃，术后28 天 和56 天时观察药物对瘢痕增生指数(HI)的影响及光镜下瘢痕变化情况。结果：二氢青蒿素组与生理盐水组之间HI差异均有显著性意义(P0.O1)；光镜下见二氢青蒿素组成纤维细胞凋亡增加及胶原纤维的含量降低。结论：二氢青蒿素可明显抑制兔耳增生性瘢痕的形成及瘢痕组织增生。 [关键词]二氢青蒿素；增生性瘢痕；兔耳瘢痕模型 [中图分类号]R619+.9[文献标识码]A[文章编号]1008-6455（2008）03-03 Effects of dihydroartemisinin on the hypertrophic scar in rabbits ears FENG Deng-chao1,2,HE Guang-zhao1,JIANG Chuan1,ZHANG Wei1,HUANG Chong-ben1 (1.Department of Plastic and Burning Surgery，the First Affiliated Hospital, Chongqing Medical University，Chongqing 400016, China;2. Department of Plastic and Burning Surgery，the Affiliated Hospital,Yan’an University, Yan’an 700016,Shaanxi，China) Abstract：Objective To investigate the effects of dihydroartemisinin on the hypertrophic scar through hypertrophic scarring of rabbits ears model． Methods Sixteen New Zealand white rabbits were used to establish the models of hypertrophic scar in ears. The rabbits in the experimental and control groups were received each 10 ml dihydroartemisinin by perfusion in dose of 180g/kg and 0g/kg (normal saline) after epithelization．At day 28 and 56 the rabbits were killed to observe hypertrophic index(HI) and morphological change of scars．ResultsCompared with the control group， dihydroartemisinin significantly reduced HI (P0.01). The proliferation of fibroblast was obviously inhibited and the contents of collagen fiber were decreased in the dihydroartemisinin treated group under light microscope on postoperative 28th and 56th day.ConclusionDihydroartemisinin can inhibit formation of hypertrophic scar in rabbit ears. Key words:dihydroartemisinin;hypertrophic scar；hypertrophic scar of rabbits ears model 增生性瘢痕(Hypertrophic scars，HS )是一种常见的病理性愈合现象，常发生于深II度烧伤或外伤后因各种原因延期愈合的创面。目前对于增生性瘢痕的治疗方法较多,用于防治瘢痕的药物研究也非常多,但由于对增生性瘢痕的形成机理尚不完全明了，临床疗效还不尽如人意。近年来发现青蒿素有较好的抑制瘢痕成纤维细胞增殖的作用，但作用机制不详。本研究旨在通过兔耳瘢痕模型的建立探讨二氢青蒿素对兔耳瘢痕形成的影响，为瘢痕防治提供实验工具和药物临床应用提供理论依据。