加味交泰汤对高血压失眠大鼠动脉内皮影响.doc

加味交泰汤对高血压失眠大鼠动脉内皮影响（1.浙江中医药大学附属第二医院，浙江 杭州 310005；2.河南中医学院，河南 郑州 450008； 3.衢州职业技术学院医学系，浙江 衢州 310053） 摘 要：目的：观察加味交泰汤对高血压失眠大鼠主动脉内皮血管性假血友病因子（Von Willebrand factor，vWF）和内皮型一氧化氮合成酶（endothelial，eNOS）表达的影响,探讨中医药防治高血压合并失眠状态思路。方法：24只12周龄SHR分为4组：空白组（高血压），模型组（高血压失眠），加味交泰汤组，归脾汤组（对照组）。采用药物睡眠剥夺法建立模型（空白组除外），用药2周后，采用免疫组化法检测主动脉eNOS、VWF8的表达。结果：加味交泰汤组eNOS表达升高，vWF表达降低显著。结论：加味交泰汤具有降压安神和明显内皮保护作用。 关键词：高血压；失眠； 交通心肾 中图分类号：R285.5文献标识码：A文章编号：1673-7717（2011）03-0571-03 The Effect of Jiaweijiaotaitang to Artery Endothelium in the Hypertension Rats with Insomnia DOU Li-ping1,YIN Zi-jie2,YAO Xiao-tian3,HUANG Shu-wei1,CHENG Zhi-qing1 （1.Zhejiang Medical University Second Affiliated Hospital, Hangzhou 310053, Zhejiang,China; 2.Henan University of TCM,Zhengzhou 450008,Henan,China; 3.Quzhou College of Techology, Quzhou 324000,Zhejiang，China） Abstract:Objective:To study the effect that Jiaweijiaotaitang to the expression of aortic endothelial von Willebrand factor （Von Willebrand factor, vWF） and endothelial nitric oxide synthase （endothelial, eNOS） in the hypertension rats with insomnia, and to explore the treatment and prevention by Traditional Chinese Medicine in the status of hypertension with insomnia. Methods: 24 SHR were divided into 4 groups of 12 weeks old, Control group,Model group, Jiaweijiaotaitang group and Guipitang group. Insomnia model rat was established by Drug sleep deprivation method （except the blank group）. After 2 weeks of treatment, detect the aortic eNOS, VWF8 expression with the method of immunohistochemistry. Results: Jiaweijiaotaitang group increased expression of eNOS, vWF expression decreased significantly. Conclusion: Jiaweijiaotaitao have significant endothelial protective effect with the antihypertensive effect and Calm the nerves. Key words:hypertension ;insomnia;imbalance between heart-yang and kidneyyin 流行病学调查显示高血压合并失眠较为普遍［1-2］。高血压合并失眠增加了心血管病不良预后的危险性［3-4］。安眠西药虽然可以改善睡眠质量，但因为存在不同程度的副作用，只适合于小剂量间断使用［5］。开发既能够改善睡眠质量又有助于改善高血压患者预后的纯中药制剂有一定的