化瘀通络方对局灶性脑缺血大鼠再灌注(MCAO)后脑组织氧自由基及NOS表达影响.doc

化瘀通络方对局灶性脑缺血大鼠再灌注(MCAO)后脑组织氧自由基及NOS表达影响（1.南京中医药大学附属医院，江苏 南京 210029；2.南京中医药大学，江苏 南京 210029） 摘 要：目的：观察化瘀通络方治疗大鼠局灶性脑缺血－再灌注损伤对脑组织自由基及一氧化氮合成酶（NOS）和iNOS变化的影响。方法：制备大鼠局灶性脑缺血再灌注损伤模型，给予化瘀通络方治疗后观察丙二醛（MDA）、超氧化物歧化酶（SOD）及一氧化氮合成酶（NOS）和诱导型一氧化氮合成酶（iNOS）的变化。结果：化瘀通络方可显著降低MDA含量以及iNOS和NOS活性，SOD含量升高。结论：在脑缺血再灌注损伤后, 化瘀通络方能抑制脑缺血再灌注后脂质过氧化反应，促进自由基清除，对抗自由基损伤，提高脑组织自身抗氧化能力，降低NOS和iNOS介导脑缺血－再灌注时的神经损伤，保护脑细胞。 关键词：化瘀通络方；脑缺血-再灌注损伤模型；SOD；MDA；NOS；iNOS 中图分类号：R285.5文献标识码：A文章编号：1673-7717（2011）03-0509-03 Effect of Huayu Tongluo Fang on the Expression of ROS and NOS in Brain Tissue During the Reperfusion After Middle Cerebral Artery Occlusion in Rats QIU Zhao-juan1，ZHU Xuan-xuan1，DA Qing-guo1，WU Xu-tong1-ZHU Wu-yuan2 （1.Affiliated Hospital of Nanjing University of Chinese Medicine，Nanjiang 210029,Jiangsu,China; 2.Nanjing University of Chinese Medicine, Nanjing 210029,Jiangsu,China） Abstract:Objective:To observe the effect of huayutongluofang on expression of ROS,NOS and iNOS during the reperfusion after middle cerebral artery occlusion in rats. Methods:The cerebral IR rats model were established by middle cerebral artery occlusion.Treated the cerebral IR rats with huayutongluofang,then observing the changes of MDA,SOD,NOS and iNOS in brain tissue. Results:Huayutongluofang could reduce the expression of MDA,iNOS and NOS in brain tissue.While, after huayutongluofang treatment,the SOD level in brain tissue was increased.Conclusions:During the reperfusion after middle cerebral artery occlusion,huayutongluofang can restrain the Lipid Peroxidation,promote removal of oxygen free radicals, compete oxygen-free radical injury. While,runhoukaiyinkeli could reduce the possibility of nerve damage,protect neural cells from injured.省略。 缺血性脑卒中（Ischemic Cerebral Stroke, ICS），中医又称缺血性脑中风，是一种突然起病的脑部血液循环障碍性疾病。祖国医学将其列为“风、痨、臌、膈”四大疑难病之首。根据WHO公布的有关数据显示，世界范围内ICS的发病率为150～200人／10万人口。而在我国，60岁以上老年人ICS发病率高达30%～45%，幸存者中约50%留有不同程度的后遗症，且复发率高达40%～60%。ICS在临床表现以猝然昏扑导致口眼歪斜、半身不遂、智力障碍为主要特征，严重影响患者生活质量，已成为前3位的疾病死因之一，其