强脉冲光对成纤维细胞增殖及周期影响.docVIP

强脉冲光对成纤维细胞增殖及周期影响[摘要]目的：研究强脉冲光对正常离体人皮肤成纤维细胞（fibroblast,FB）的生长活力、细胞增殖能力以及细胞周期变化的影响并探讨其可能的发生机制,为强脉冲光除皱提供可能的理论依据。方法：培养人皮肤成纤维细胞，应用MTT比色法、免疫组织化学的方法和流式细胞仪（FCN）分析技术，观察皮肤成纤维细胞经不同能量的强脉冲光照射后细胞的生长活力、细胞增殖能力以及细胞周期变化。结果：强脉冲光能促进体外培养人皮肤成纤维细胞增殖，FCN结果显示强脉冲光照射后，成纤维细胞G1期百分比降低，而S期百分比升高，增殖指数PrI值（S+G2M）百分比增高。结论：强脉冲光可促进正常成纤维细胞增殖和DNA合成，从而为强脉冲光面部美容除皱供理论依据。 [关键词]强脉冲光；成纤维细胞；细胞增殖； 细胞周期； 面部除皱 [中图分类号]Q813.1[文献标识码]A[文章编号]1008-6455（2007）10-1331-03 The effects of intense pulse light（IPL） on the cell proliferation activity and cell cycle of human fibroblasts LI Xiao-ge,LI Shi-rong,CAO Chuan,DENG Xiang-dong (Department of Plastic Surgery,Southwest Hospital of the Third Military Medical University,Chongqing 400038,China) Abstract:ObjectiveTo investigate the effects of intense pulse light on the cell proliferation and cell cycle of human fibroblasts and to provide the possible theoritical basis for the treatment of face-lift.MethodsCultured human skin fibroblasts and treated with different dose of intense pulse light radiation.By culturing 24 hours analyzed cell cycle with flow cytometr（FCM）, the cell proliferation activity was measured by MTT assay.ResulstsResults showed both proportion of cells in S and G2M phase was increased and that of G1 was decreased when the cells were irradiate with IPL in comparison with non- irradiate significantly, PrI were beyond its normal range.ConclusionIntense pulse light radiation could promote the cell proliferation and synthesis of DNA in cultured primary fibroblasts. Key words: intense pulse light; fibroblast ;PCNA; cell cycle; face-lift 皮肤衰老是人体老化的外部表现，其中成纤维细胞在皮肤衰老过程中扮演着重要角色，在皮肤老化时成纤维细胞的生长活力降低，甚至出现明显的生长间隙阻滞，G2M期细胞数增加。本实验通过研究强脉冲光对体外培养的成纤维细胞增殖能力、细胞周期变化以及细胞增殖核抗原的影响，为强脉冲光在面部老化治疗中的应用提供理论依据。 1资料和方法 1.1 标本、试剂及所用仪器:标本选择泌尿外科身体健康的中青年的包皮手术时切除的包皮。实验地点为解放军第三军医大学西南医院。细胞培养、实验指标检测均于环境设施为清洁级、室温20℃～25℃、相对湿度为60％～70％条件下进行。光子嫩肤仪（GP666－B，深圳市金威源科技有限公司），DMEM培养基（hyclon，美国），新生小牛血清（GibDD，美国），分组：正常对照组采用的DMEM培养液内含 10ml／L小牛血清，HEPES 24g／L，碳酸氢钠15g／L青霉素 100U／ml，链霉素 100