非显微外科手术建立小鼠颈动脉损伤模型.pdfVIP

34 Chin J A口口1 physioI2008;24 (1) EFFECTS OF HYPOXIC PRECONDITIONING ON HYPOXIA TOLERANCE OF ASTROCYTES IN VITRO ZHOU Xuan 1 , ZHANG Hai-fu 1, LIU Fa-yi 1, HE Fang 1, YN叫G Li-juan 1 , HUANG Bai-shengl , WU Li-xianglA , WEI Wei2 (1. Department of Physiology , Basic Medical Sch∞1， 2. General Surgery Department , Xi田哩ya Hospital , Central South University , Changsha 410078 , China) AffiTRACf Aim: To explore the mechanisms of hypoxic pre∞nditioning on protecting cultured astrocytes from hypoxia i均町y.Meth创Is: Cultured astr∞ytes were divided randomly into several groups: control (C) , hypoxia (H) and hypoxic pre∞nditiollÌng( HP). Cells MTT metabolic activity , qualitation of apoptosis and modality to explore the protection effects of hypoxic preconditioning. Immuno- cyt∞hemistry of BcI-2 and Bax to explore the mechanisms of hypoxic preconditiollÌng on protecting astr，∞ytes from hy归，xia. Results: Com阳red with H group there was marked increase of MTT metabolic activity in HP48 and HP72 groups. Immunocyt∞hemistry of BcI-2 and Bax showed that ∞mpared with H group , expression of BcI-2 was increased in HPgroup ,while expr臼sion of Bax was de- creased in HP group. Conclusion: H只汩汩c pre∞nditiollÌng can protect astr∞ytes from h只)()xia. 臼le possible mechanism maybe con- cemed with inhibition of Bax and maintain of BcI-2 to depr岱s apoptosis pr∞edure. KEY WORDS: hypoxic preconditioning; astr，∞ytes; hypoxia; apoptosis 非显微外科手术建立小鼠颈动脉损伤模型祷 尹扬光1 ，黄岚14 ，赵晓辉1 ，周健1 ，方玉强2 ，吴楠3 (1.第三军医大学新桥医院心内科，重庆 400037; 2. 第三军医大学大坪医院心内科，重庆 400042; 3. 第三军医大学新桥医院眼科，重庆 400037) 摘要 目的:探讨以非显微外科方法建立小鼠颈动脉