实验大鼠斜方肌肌纤维型组成及分布研究.pdfVIP

解剖科学进展 Progress of Anatomical Sciences 2008．14(1)：51～53 实验大鼠斜方肌肌纤维型组成及分布研究 章 涛 ，张 潜 ，王正义 ，薛 黔 ，陈代雄 (遵义医学院1．附属医院贵州省细胞工程重点实验室；2解剖学教研室，贵州遵义563003； 3．重庆医科大学基础医学研究所，重庆400016) 【摘要】目的 探讨大鼠斜方肌的肌纤维型组成和分布，借以了解该肌功能。方法 取成年sD大鼠斜方肌 升部肌组织进行冰冻切片(8 m厚)，采用Guth—Samaha肌球蛋白ATP酶组织化学染色法并稍做改良，对其进行 肌纤维分型研究。结果 SD大鼠斜方肌经肌球蛋白ATP酶组织化学染色后可明确分出2种肌纤维型，即白色的I 型纤维 (慢缩纤维)和深褐色的Ⅱ型纤维(快缩纤维)，2种肌纤维在肌内呈棋盘样均匀分布；图像分析计数其 I型纤维占(48．8±6．9)％，II型纤维比例为 (51．2±6．9)％，2者比较差异无统计学意义 (P0．05)。结论 SD大鼠斜方肌2型肌纤维所占比例均等，与其维持颈背部姿势的作用一致。 【关键词】 斜方肌；肌纤维型；肌球蛋白ATp酶；大鼠 【中图分类号】Q959．837；Q954．54 8 【文献标识码】A 【文章编号】1006—2947(2008)01—0051—03 Composition and Distribution of Muscle Fiber Types of Rat Trapezius Muscle ZHANG Tao ，ZHANG Qian ，WANG Zheng-Yi ，XUE Qian ，CHEN Dai—xiong (1．Key Laboratory of Cell Engineering of Guizhou Province，The Affiliated Hospital；2．Department of Anatomy，Zunyi Medical College，Guizhou Zunyi 563003；3．Institute of Basic Medicine，Chongqing Medical University，Chongqing 400016 China) 【Abstract】objective To investigate the composition and distribution of fiber types of SD rat trapezius muscle and evaluate the muscle function．Methods The ascending portion of trapezius muscles from both sides of 10 adult SD rats was studies．8一um thick cryostat sections were cut and then stained with the myosin-ATPase by Guth Samaha following a procesure modified partially．Results Trapezius muscles of SD rats can be clearly identified as type I(slow twitch oxidative fibers，SO)and type II(fast twitch glycolytic fibers，FG)，the former looks brightly while the latter darkly．The distribution pattern of this two types of muscle fibers is uniform and chessboard-like．The percentage of type I fibers Was(48．8±6．9)％， much the same as the type II fibers(5 1，2土6，9)％(P0．05)．Conclusion Two types of fibers w