游离锌离子在APP／PS1转基因小鼠嗅球内的定位.pdfVIP

解剖科学进展 Progress of Anatomical Sciences 2008 ,14(1):36-38, 42 游离铸离子在APP/PS1 转基因小鼠嗅球内的定位 张丽红112 ，王辛1 ，郑均1 ，于丹1 ，荣明1 ，王占友严 ( 1.中国医科大学基础医学院组织胚胎学教研室，沈阳11αlOl; 2.辽宁中医药大学基础医学院组织胚胎学教研室，沈阳11∞32 ) [摘要]目的研究铸离子在APPIPSI转基因小鼠嗅球内的定位，为铸离子参与阿尔茨海默病发病的相关性 研究提供重要的形态学依据。方法应用浸入式金属自显影技术 (AMG) 检测铸离子在野生型小鼠和APPIPSI转 基因小鼠嗅球内的分布。结果在野生型小鼠和APPIPSI转基因小鼠嗅球内， AMG反应产物呈棕黑色，嗅球的5层 结构清晰可见，其中颗粒层铸离子含量最高。在APPIPSI转基因小鼠嗅球内，还可见铸离子聚集在老年斑内， AMG阳性的老年斑主要分布于含镑量较高的颗粒层，其它各层仅见散在分布。野生型小鼠嗅球内未见AMG阳性 的老年斑。结论 APPIPSI转基因小鼠嗅球的老年斑内含有大量的铸离子，提示铸离子在嗅球Aß 老年斑的形成 过程中发挥重要作用，参与AD的发病和进展。 [关键词]铸离子:嗅球;老年斑; APPIPSI [中图分类号] R322.81 [文献标识码] A [文章编号] 1∞6-2947 (2∞8) 01-∞36-03 Localization of Free Zinc Ions in the Olfactory Bulb of APP/PS 1 Transgenic Mouse Brain ZHANG Li-hong.2 , WANG Xin , ZHENG Wei , YU Dan , RONG Ming , WANG Zhan-you (1 Department ofHìstology and Emhryology, China Medical University , Shenyang 11αlOl ， China; 2 Department of Histology and Embryology,Liaoning Unìversity of Traditional Chinese Medicìne ,Shenyang 11∞32， China) [ Abstract] Objective To ìnvestigate the localìzatìon of free zìnc ìons ìn the olfactory bulb of APPIPSI transgenìc mouse braìn and to provide a morphological evidence for zinc ìnvolvìng in the pathological process of Alzheìmers dìsease. Method Immersìon autometallography was used to detect the distrìbution of free zìnc ìons ìn the wìld-type and APPIPSI 位ansgenic mouse olfactory bulb. Results In the olfactOlγbulb of wìld-type and APPIPSI transgenic mìce , dark brown AMG products were found in all five layers and predominately in the granule celllayer. In the olfactory bulb of APPIPSI transgenic mouse , zìnc ions were accumulated in the senile plaques which were mainly distrìbuted in the gra