藻酸盐凝胶支架在生物学修复椎间盘退行性变中的应用.pdfVIP

中国组织工程研究与临床康复 J2眷 J 2008一Ol—Ol出版 JournalofClinicalRehabilitativeT~sueEngineeringResearch January 1．2008 Vo1．12,No．1 ；l} 麴 藻酸盐凝胶支架在生物学修复椎间盘退行性变中的应用☆ 赵 鑫，黄 师，严 宁，刘超乾，侯铁胜 Alginate scaffold in the repair of lumbar intervertebral degenerative disc by biological method Zhao Xin。Huang Shi。Yan Ning。Liu Chao-qian，Hou Tie-sheng Abstract Department of AIM：Alginate gel call be used in cell culture in vitro and tissue repair in vivo．This study observed the efrect of bone marrow Orthopedics，Chang- mesenchymal stem cells(BMSCs)co—cultured with alginate gel on repairing the degeneration oflumbar intervertebral disc． hai Hospital of METH0DS：111e experiment was performed at the laboratory of Department of Orthopedics．Changhai Hospital of Second Military Second Military Medical University of Chinese PLA from March 2006 to March 2007．①BMSCs were isolated by density gradient centrifugation Medical University of Chinese PLA． and cultured with 1．2％ alginate solution in vitro．Cell growth was observed．( Models of degenerative intervertebral disc were Shanghai 200433， established in eighteen New Zealand rabbits and the animals were randomly divided into three groups：in treatment group．BMSCs China encapsulated in alginate beads were transplanted into the degenerative disc；in control group，only BMSCs were transplanted；in blank group，no cells were transplanted．③111e changes in intervertebral disc height and T2 signal were det