肾上腺切除大鼠冲击伤后天然免疫功能的变化及其与模式识别受体的关系.doc

肾上腺切除大鼠冲击伤后天然免疫功能的变化及其与模式识别受体的关系杨策周琳琳严军王海燕周建云程英刘庆崔莉蒋建新 目的调控。本研究通过。方法Sprague-Dawley成年大鼠在双侧肾上腺切除后7天，遭受中度冲击伤，观察外周血白细胞数目、腹腔巨噬细胞吞噬大肠杆菌以及肝脏、肺脏和肠系膜淋巴结组织细菌移位以及全血、肺组织肿瘤坏死因子（TNF）α的变化，进而检测肺组织SR-A、CD14，TLR4 和MD2 mRNA 的表达。结果肝、肺淋巴结TNF-α分泌在肾上腺切除后也进一步增强。肺组织SR-A、CD14、TLR4和MD2的mRNA在肾上腺切除后显著减少，并伴有显著的炎症反应和组织损伤。结论SR-A、CD14、TLR4和MD2表达密切相关，对于防止伤后感染中图分类号：F275.1文献标识码：Affects of adrenalectomy on innate immune responses following blast injury in rats YANG Ce, Yan Jun, ZHOU Lin-lin, WANG Hai-Yan, ZHOU Jian-Yun, LIU Qin, CHEN Ying, JIANG Jian-xin (State Key Laboratory of Trauma, Burns and Combined Injury, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China) Abstract：Objective The regulation of neuroendocrine hormones on the innate immune responses remains controversial. This report investigated whether the adrenal-derived hormones directly enhance the innate immune responses and further study its relation to main pattern recognition receptors following trauma. Method Bilateral adrenalectomy was performed on Sprague-Dawley adult rats, followed by mild blast wave injury. Peripheral blood leukocyte counting, macrophage phagocytosis, bacterial translocation (BT) in lung tissues, liver tissues and mesenteric lymph nodes (MLN), tumor necrosis factor (TNF) alpha levels in blood samples and lung tissues, and mRNA expression for scavenger receptor (SR) A, CD14, Toll like receptor (TLR) 4 and MD2 were assessed. Results After blast injury, the number of peripheral leukocytes was decreased and the phagocytosis of peritoneal macrophages was increased, which were both reversed by adrenalectomy. The gut-derived BT, TNF-α secretion and the blood sensitivity to lipopolysaccharide (LPS) challenge were elevated in adrenalectomized rats. Furthermore, the mRNA levels of SR-A, CD14, TLR4 and MD2 in lung tissues of adrenalectomized rats all decreased significantly. Adrenalectomized rats showed enhancement of inflammatory responses and severe tissue injuries. Con