effects of neonatal systemic inflammation on blood-brain barrier permeability and behaviour in juvenile and adult rats新生儿全身炎症对血脑屏障通透性的影响,在青少年和成年大鼠的行为.pdfVIP

Hindawi Publishing Corporation Cardiovascular Psychiatry and Neurology Volume 2011, Article ID 469046, 10 pages doi:10.1155/2011/469046 Research Article Effects of Neonatal Systemic Inflammation on Blood-Brain Barrier Permeability and Behaviour in Juvenile and Adult Rats H. B. Stolp,1, 2 P. A. Johansson,1, 3 M. D. Habgood,1 K. M. Dziegielewska,1 N. R. Saunders,1 and C. J. Ek1 1 Department of Pharmacology, University of Melbourne, Medical Building 181, Parkville VIC 3010, Australia 2 Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford OX13QX, UK 3 Institute of Stem Cell Research, Helmholtz Zentrum M¨unchen, 85764 Neuherberg, Germany Correspondence should be addressed to C. J. Ek, cjek@.au Received 11 August 2010; Revised 9 January 2011; Accepted 18 January 2011 Academic Editor: Alon Friedman Copyright © 2011 H. B. Stolp et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Several neurological disorders have been linked to inflammatory insults suffered during development. We investigated the effects of neonatal systemic inflammation, induced by LPS injections, on blood-brain barrier permeability, endothelial tight junctions and behaviour of juvenile (P20) and adult rats. LPS-treatment resulted in altered cellular localisation of claudin-5 and changes in ultrastructural morphology of a few cerebral blood vessels. Barrier permeability to sucrose was significantly increased in LPS treated animals when adult but not at P20 or earlier. Behavioural tests showed that LPS treated animals at P20 exhibited altered behaviour using prepulse inhibition (PPI) analysis, whereas adults demonstrated alter