dynamics of spreading and alignment of cells cultured in vitro on a grooved polymer surface扩散动力学和对齐的细胞在体外培养槽聚合物表面.pdfVIP

Hindawi Publishing Corporation Journal of Nanomaterials Volume 2011, Article ID 413079, 10 pages doi:10.1155/2011/413079 Research Article Dynamics of Spreading and Alignment of Cells Cultured In Vitro on a Grooved Polymer Surface Thomas Peterbauer,1, 2 Sergii Yakunin,1 Jakub Siegel,1 Steffen Hering,1 Marc Fahrner,3 Christoph Romanin,3 and Johannes Heitz1 1 Institute of Applied Physics, Johannes Kepler University Linz, 4040 Linz, Austria 2 Department of Pharmacology and Toxicology, University of Vienna, 1090 Vienna, Austria 3 Institute of Biophysics, Johannes Kepler University Linz, 4040 Linz, Austria Correspondence should be addressed to Johannes Heitz, Johannes.heitz@jku.at Received 21 April 2010; Revised 28 June 2010; Accepted 7 July 2010 Academic Editor: Lu Sun Copyright © 2011 Thomas Peterbauer et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. We used mechanically embossed polyester films to analyze the dynamics of cell alignment and cell-specific factors modulating the response of Chinese hamster ovary (CHO) cells and of a rat myogenic cell line to the surface topography. The films used had grooves with a periodicity of approximately 750 nm and a depth of 150 nm. Both cell lines responded to the topographical feature. On unpatterned control areas, cells of both lines showed a random distribution with orientation angles close to 45◦ . Both cell types exhibited an elongated morphology on the patterned surface. CHO cells typically showed bipolar spreading. Their contact area increased almost exclusively along the groove direction. Likewise, freshl