biphasic activation of nuclear factor-kappa b in experimental models of subarachnoid hemorrhage in vivo and in vitro两相的激活核factor-kappa b在蛛网膜下腔出血实验模型的体内和体外.pdfVIP

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biphasic activation of nuclear factor-kappa b in experimental models of subarachnoid hemorrhage in vivo and in vitro两相的激活核factor-kappa b在蛛网膜下腔出血实验模型的体内和体外.pdf

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biphasic activation of nuclear factor-kappa b in experimental models of subarachnoid hemorrhage in vivo and in vitro两相的激活核factor-kappa b在蛛网膜下腔出血实验模型的体内和体外

Hindawi Publishing Corporation Mediators of Inﬂammation Volume 2012, Article ID 786242, 10 pages doi:10.1155/2012/786242 Research Article Biphasic Activation of Nuclear Factor-Kappa B in Experimental Models of Subarachnoid Hemorrhage In Vivo and In Vitro Wan-Chun You,1 Wei Li,1 Zong Zhuang,1 Yong Tang,1 Hu-Chen Lu,1 Xiang-Jun Ji,1 Wei Shen,2 Ji-Xin Shi,1 and Meng-Liang Zhou1 1 Department of Neurosurgery, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, Jiangsu, Nanjing 210002, China 2 Department of Neurosurgery, Jinling Hospital, School of Medicine, Southern Medical University (Guangzhou), 305 East Zhongshan Road, Jiangsu, Nanjing 210002, China Correspondence should be addressed to Ji-Xin Shi, shijx52@ and Meng-Liang Zhou, mengliangzhou@ Received 12 July 2012; Accepted 21 August 2012 Academic Editor: Dennis Daniel Taub Copyright © 2012 Wan-Chun You et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. It has been proven that nuclear factor-kappa B (NF-κB) is activated as a well-known transcription factor after subarachnoid hemorrhage (SAH). However, the panoramic view of NF-κB activity after SAH remained obscure. Cultured neurons were signed into control group and six hemoglobin- (Hb-) incubated groups. One-hemorrhage rabbit SAH model was produced, and the rabbits were divided randomly into one control group and ﬁve SAH groups. NF-κB activity was detected by electrophoretic mobility shift assay (EMSA) and immunohistochemistry. Real-time polymerase chain reaction (PCR) was performed to assess the downstream genes of NF-