biochemical evidence for the presence of two α-glucoside abc-transport systems in the hyperthermophilic archaeon pyrococcus furiosus生化证据的存在两个α-glucoside abc-transport系统hyperthermophilic archaeon海床furiosus.pdfVIP

Archaea 1, 19–25 © 2002 Heron Publishing—Victoria, Canada Biochemical evidence for the presence of two -glucoside ABC- transport systems in the hyperthermophilic archaeon Pyrococcus furiosus 1 1 1,2 SONJA M. KONING, WIL N. KONINGS and ARNOLD J. M. DRIESSEN 1 Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, P. O. Box 14, 9750 AAHaren, The Netherlands 2 Author to whom correspondence should be addressed (a.j.m.driessen@biol.rug.nl) Received June 14, 2001; accepted September 14, 2001; published online October 11, 2001 Summary The hyperthermophilic archaeon Pyrococcus al. 1998, Greller et al. 1999). This system mediates the uptake furiosus can utilize different carbohydrates, such as starch, of trehalose and maltose but not of maltodextrins. In contrast, a maltose and trehalose. Uptake of α-glucosides is mediated by single maltose, trehalose and maltodextrin transporter has two different, binding protein-dependent, ATP-binding cas- been described for the thermophilic bacterium Thermoanaero- sette (ABC)-type transport systems. The maltose transporter bacter ethanolicus (Jones et al. 2000). The genome of P. furi- also transports trehalose, whereas the maltodextrin transport osus contains a gene cluster that encodes an ABC-type system mediates the uptake of maltotriose and higher malto- transporter that is nearly identical to the trehalose/maltose oligosaccharides, but not maltose. Both transport systems are transporter of T. litoralis (DiRuggiero et al. 2000). In addition, induced during growt