different residues on the surface of the methanothermobacter thermautotrophicus mcm helicase interact with single- and double-stranded dna不同表面残留methanothermobacter thermautotrophicus mcm解旋酶与单-和双链dna.pdfVIP

Hindawi Publishing Corporation Archaea Volume 2010, Article ID 505693, 8 pages doi:10.1155/2010/505693 Research Article Different Residues on the Surface of the Methanothermobacter thermautotrophicus MCM Helicase Interact with Single- and Double-Stranded DNA Nozomi Sakakibara,1, 2 Rajesh Kasiviswanathan,1, 3 and Zvi Kelman4 1 Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, MD 20850, USA 2 Laboratory of Viral Diseases, NIAID, NIH, 4 Center Drive, Bethesda, MD 20892, USA 3 Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, 111 TW Alexander Drive, Research Triangle Park, NC 27709, USA 4 Department of Cell Biology and Molecular Genetics, Institute for Bioscience and Biotechnology Research, University of Maryland, 9600 Gudelsky Drive, Rockville, MD 20850, USA Correspondence should be addressed to Nozomi Sakakibara, sakakibaran@ Received 5 August 2010; Accepted 16 October 2010 Academic Editor: Y. Ishino Copyright © 2010 Nozomi Sakakibara et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The minichromosome maintenance (MCM) complex is thought to function as the replicative helicase in archaea, separating the two strands of chromosomal DNA during replication. The catalytic activity resides within the C-terminal region of the MCM protein, while the N-terminal portion plays an important role in DNA binding and protein multimerization. An alignment of MCM homologues from several archaeal species revealed a number of conserved amino acids. Here several of the conserved