evaluation of the morphology and osteogenic potential of titania-based electrospun nanofibers的形态和成骨的潜力评价titania-based静电纺丝.pdfVIP

Hindawi Publishing Corporation Journal of Nanomaterials Volume 2012, Article ID 959578, 7 pages doi:10.1155/2012/959578 Research Article Evaluation of the Morphology and Osteogenic Potential of Titania-Based Electrospun Nanofibers Xiaokun Wang,1 Jingxian Zhu,2 Ling Yin,1 Shize Liu,1 Xin Zhang,2 Yingfang Ao,2 and Haifeng Chen1 1 Department of Biomedical Engineering, College of Engineering, Peking University, No. 5 Yiheyuan Road, Haidian District, Beijing 100871, China 2 Institute of Sports Medicine, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, China Correspondence should be addressed to Haifeng Chen, haifeng.chen@ Received 8 February 2012; Accepted 3 April 2012 Academic Editor: Krasimir Vasilev Copyright © 2012 Xiaokun Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Submicron-scale titania-based ceramic fibers with various compositions have been prepared by electrospinning. The as-prepared nanofibers were heat-treated at 700◦C for 3 h to obtain pure inorganic fiber meshes. The results show that the diameter and morphology of the nanofibers are affected by starting polymer concentration and sol-gel composition. The titania and titania- silica nanofibers had the average diameter about 100–300 nm. The crystal phase varied from high-crystallized rutile-anatase mixed crystal to low-crystallized anatase with adding the silica addition. The morphology and crystal phase were evaluated by SEM and XRD. Bone-marrow-derived mesenchymal stem cells were seeded on titania-silica 50/50 fiber meshes. Cell number and early differentiation marker expressions were an