combinational effects of prebiotics and soybean against azoxymethane-induced colon cancer in vivo益生元组合的影响和大豆体内azoxymethane-induced结肠癌.pdfVIP

Hindawi Publishing Corporation Journal of Nutrition and Metabolism Volume 2011, Article ID 868197, 9 pages doi:10.1155/2011/868197 Research Article Combinational Effects of Prebiotics and Soybean against Azoxymethane-Induced Colon Cancer In Vivo V. P. Gourineni,1 M. Verghese,1, 2 J. Boateng,1 L. Shackelford,1 N. K. Bhat,3 and L. T. Walker2 1 Nutrition Biochemistry and Carcinogenesis Laboratory, Department of Food and Animal Sciences, Alabama AM University, Normal, AL 35762, USA 2 Department of Food Science, Alabama AM University, P.O. Box 1628, Normal, AL 35762, USA 3 Department of Chemistry, Alabama AM University, Normal, AL 35762, USA Correspondence should be addressed to M. Verghese, martha.verghese@ Received 22 January 2011; Revised 18 April 2011; Accepted 28 May 2011 Academic Editor: M. Pagliassotti Copyright © 2011 V. P. Gourineni et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Prebiotic fructans are nondigestible carbohydrates with numerous health benefits. Soybean is a rich source of phytonutrients such as isoflavones. The objective of this study was to evaluate the chemopreventive effects of prebiotics (Synergy1) and soybean meal (SM) at 5% and 10% levels alone and in combination on azoxymethane- (AOM-) induced colon carcinogenesis. After one wk of acclimatization, Fisher 344 male rats (N = 90) were randomly assigned to 9 groups (n = 10). Control rats (C) were fed AIN- 93G/M. Two s/c injections of AOM were administered to rats at 7 and 8 wk of age at 16 mg/kg body weight. Rats were killed by CO2 asphyxiation at 45 wk. Tumor incidence (%) in treatment groups ranged from 40 to 75 compared to