direct quantitative detection and identification of lactococcal bacteriophages from milk and whey by real-time pcr application for the detection of lactococcal bacteriophages in goats raw milk whey in france英文论文.pdfVIP

Hindawi Publishing Corporation International Journal of Microbiology Volume 2011, Article ID 594369, 9 pages doi:10.1155/2011/594369 Research Article Direct Quantitative Detection and Identification of Lactococcal Bacteriophages from Milk and Whey by Real-Time PCR: Application for the Detection of Lactococcal Bacteriophages in Goat’s Raw Milk Whey in France ¨ ´ Mai Huong Ly-Chatain, Loıc Durand, Veronique Rigobello, Annabelle Vera, and Yann Demarigny Department of Food Industry and Quality, ISARA-Lyon, 23 Jean Baldassini, 69364 Lyon Cedex 07, France Correspondence should be addressed to Mai Huong Ly-Chatain, mhchatain@isara.fr Received 4 April 2011; Accepted 12 July 2011 Academic Editor: Giuseppe Comi Copyright © 2011 Mai Huong Ly-Chatain et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The presence of Lactococcus bacteriophages in milk can partly or completely inhibit milk fermentation. To prevent the problems associated with the bacteriophages, the real-time PCR was developed in this study for direct detection from whey and milk of three main groups of Lactococcus bacteriophages, c2, 936, and P335. The optimization of DNA extraction protocol from complex ma- trices such as whey and milk was optimized allowed the amplification of PCR without any matrix and nontarget contaminant interference. The real-time PCR program was specific and with the detection limit of 102 PFU/mL. The curve slopes were −3.49, 2 −3.69,