gene silencing of e-selectin block recruitment of endothelial progenitor cell to vascular endothelium under flow基因沉默e-selectin块招聘的内皮祖细胞血管内皮在流.pdfVIP

J. Biomedical Science and Engineering, 2010, 3, 550-555 JBiSE doi:10.4236/jbise.2010.36077 Published Online June 2010 (http://www.SciRP.org/journal/jbise/). Gene silencing of E-selectin block recruitment of endothelial progenitor cell to vascular endothelium under flow Sunil Sharma, Masayuki Yoshida Life Science and Bioethics Research Center, Tokyo Medical and Dental University, Tokyo, Japan. Email: masavasc@tmd.ac.jp Received 16 February 2010; revised 20 March 2010; accepted 25 March 2010. ABSTRACT vated to capture progenitor cells in regions where endo- thelium regeneration is needed. Short interfering RNA (siRNA) is a powerful tech- Though ischemia is believed to be the physiological nique that can suppress gene expression in a variety stimulus for EPC mobilisation from the bone marrow, of cells including mammalian cells. Endothelial pro- the mechanisms of EPC recruitment are less well under- genitor cells (EPCs) are bone marrow—derived stood. haematopoietic progenitor cells that have been im- Little is understood concerning the molecular mecha- plicated in vasculogenesis. We demonstrated for the nisms of EPC adhesion to endothelial cells. In contrast, first time that gene silencing of endotheli