assessment of the microbiota in microdissected tissues of crohns disease patients微生物群在microdissected组织的评估克罗恩病的病人.pdfVIP

Hindawi Publishing Corporation International Journal of Inflammation Volume 2012, Article ID 505674, 11 pages doi:10.1155/2012/505674 Research Article Assessment of the Microbiota in Microdissected Tissues of Crohn’s Disease Patients Gert De Hertogh,1 Bart Lemmens,1 Peter Verhasselt,2 Ronald de Hoogt,2 Xavier Sagaert,1 Marie Joossens,3 Gert Van Assche,3 Paul Rutgeerts,3 Severine Vermeire,3 and Jeroen Aerssens2 1 Department of Morphology and Molecular Pathology, University Hospitals KULeuven, Minderbroedersstraat 12, 3000 Leuven, Belgium 2 Department Research Informatics Integrated Genomics, Janssen Pharmaceutical Companies of Johnson Johnson, Turnhoutseweg 30, 2340 Beerse, Belgium 3 Department of Gastroenterology, University Hospitals KULeuven, Herestraat 49, 3000 Leuven, Belgium Correspondence should be addressed to Gert De Hertogh, gert.dehertogh@uzleuven.be Received 18 July 2011; Revised 5 September 2011; Accepted 14 September 2011 Academic Editor: Derek Jewell Copyright © 2012 Gert De Hertogh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The microbiota of the gastrointestinal tract is frequently mentioned as one of the key players in the etiopathogenesis of Crohn’s disease (CD). Four hypotheses have been suggested: the single, still unknown bacterial pathogen, an abnormal overall composition of the bowel microbiota (“dysbiosis”), an abnormal immunological reaction to an essentially normally composed microbiota, and increased bacterial translocation. We propose that laser capture microdissection of selected microscopic structures, followed by