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auto-offsetting and -referencing in biomolecular nmr experimentsauto-offsetting和引用生物分子核磁共振实验.pdf

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auto-offsetting and -referencing in biomolecular nmr experimentsauto-offsetting和引用生物分子核磁共振实验

Vol.2, No.3, 208-214 (2011) Journal of Biophysical Chemistry doi:10.4236/jbpc.2011.23025 Auto-offsetting and -referencing in biomolecular NMR experiments * Shanmin Zhang Shanghai key Laboratory of Magnetic Resonance, Department of Physics, East China Normal University, Shanghai, China; * Corresponding Author: shanminz@ Received 16 April 2011; revised 1 May 2011; accepted 8 July 2011. ABSTRACT magnetic field (B ) is determined by the Zeeman (B ) 0 0 and shielding (B ) interactions [1,2]. The shielding A frequency ratio RatioXH associated with two 0 interaction is induced by the nearby electrons precessing carrier frequencies in ppm is introduced for at their own Larmor frequencies, which produce a very achieving auto-offsetting and -referencing (AOR) 1 small magnetic field at the site of the nucleus. As the in biomolecular NMR. Once the center of H is Larmor frequency of the electrons, it is also proportional referenced, all the offsets in other dimensions to B0. The shielding coefficient  is referred to as are automatically determined and