biosurfactant-producing lactobacilli screening, production profiles, and effect of medium composition堆肥乳酸杆菌的筛选、生产资料和介质组成的影响.pdfVIP

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biosurfactant-producing lactobacilli screening, production profiles, and effect of medium composition堆肥乳酸杆菌的筛选、生产资料和介质组成的影响.pdf

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biosurfactant-producing lactobacilli screening, production profiles, and effect of medium composition堆肥乳酸杆菌的筛选、生产资料和介质组成的影响

Hindawi Publishing Corporation Applied and Environmental Soil Science Volume 2011, Article ID 201254, 9 pages doi:10.1155/2011/201254 Research Article Biosurfactant-Producing Lactobacilli: Screening, Production Proﬁles, and Effect of Medium Composition ˜ ´ ´ Eduardo J. Gudina, Jose A. Teixeira, and Lıgia R. Rodrigues Institute for Biotechnology and Bioengineering (IBB), Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal ˜ Correspondence should be addressed to Eduardo J. Gudina, egudina@deb.uminho.pt Received 22 June 2011; Accepted 28 September 2011 Academic Editor: Elena Tamburini ˜ Copyright © 2011 Eduardo J. Gudina et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Biosurfactant production was screened in four lactobacilli strains. The highest biosurfactant production (excreted and cell- bound biosurfactants) was achieved with Lactobacillus paracasei ssp. paracasei A20, a strain isolated from a Portuguese dairy plant, with a decrease in the surface tension of 6.4 mN m−1 and 22.0 mN m−1, respectively. Biosurfactant production by this strain was evaluated under diﬀerent culture broth compositions. The use of diﬀerent nitrogen sources revealed that yeast extract is essential for bacterial growth, while peptone is crucial for biosurfactant synthesis. For biosurfactant production, the use of peptone and meat extract yielded a higher production when