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bod exertion and od600 measurements in presence of heavy metal ions using microbes from dairy wastewater as a seedbod努力和od600测量存在的重金属离子利用乳品废水的微生物作为种子
J. Water Resource and Protection, 2010, 2, 478-488
doi:10.4236/jwarp.2010.25055 Published Online May 2010 (http://www.SciRP.org/journal/jwarp)
BOD Exertion and OD600 Measurements in Presence of
Heavy Metal Ions Using Microbes from Dairy
Wastewater as a Seed
*
Susheel K. Mittal , Siloni Goel
School of Chemistry Biochemistry Thapar University, Patiala, India
E-mail : smittal@
Received October 12, 2009; revised December 3, 2009; accepted January 20, 2010
Abstract
BOD measurements in presence of cobalt, nickel, copper, zinc, silver and cadmium are reported using
wastewater from dairy industry as a seed. Extent of inhibition in BOD is studied for variables like, concen-
tration of metal ions (0.2 mM to 1.4 mM), pH (3 to 8) and temperature of incubation (15 ℃, 20℃, 25℃,
30℃ and 35 ℃). Results of BOD inhibition are supported by absorbance measurement (OD600) studies of
microbial matter preserved in Luriya broth medium. OD measurement results are used to derive minimum
inhibitory concentration, i.e., threshold concentration of each metal showing toxicity towards microbes. Sil-
ver is found to be the most toxic element.
Keywords: BOD Inhibition, Metal Ions, OD600, Microbes, Dairy Wastewater
1. Introduction These metals are generally transition elements and have
unfilled d-orbitals, which help the metal ions to complex
with chelating molecules like enzymes proteins and
In the normal
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