ceruloplasmin levels in human sera from various diseases and their correlation with patient’s age and gender人类各种疾病的血清和血浆铜蓝蛋白水平与患者的年龄和性别的关系.pdfVIP

Vol.1, No.2, 104-110 (2009) Health doi:10.4236/health.2009.12017 Ceruloplasmin levels in human sera from various diseases and their correlation with patient’s age and gender 1* 2 3 Viorica Lopez-Avila , William H. Robinson , Kirk Lokits 1 Agilent Technologies, Santa Clara, CA, USA; Corresponding author: viorica_lopez-avila@ 2Stanford University, Stanford, CA, USA 3University of Cincinnati, Cincinnati, OH, USA; Present address: Midwest Research Institute, 425 Volker Blvd, Kansas City, MO, USA Received 7 July 2009; revised 5 August 2009; accepted 7 August 2009. ABSTRACT Keywords: Ceruloplasmin; HPLC-ICPMS; Immunoaffinity Chromatography Ceruloplasmin (Cp), a copper metalloprotein in human serum has been a valuable diagnostic marker in Wilson’s disease where Cp levels tend 1. INTRODUCTION to be low while high levels in serum were asso- ciated with myocardial infarction, neoplastic Ceruloplasmin (Cp) is a blue alpha-2 glycoprotein with a and inflammatory conditions. There is no stan- molecular weight of 132,000 u [1]. It binds 90-95% of dardized reference method for Cp and current blood plasma copper (Cu), has 6-7 Cu ions per molecule immunologic and bichromatic assays have a (1) and exhibits ferroxidase activity [1,2], amine oxidase number of drawbacks. The method described activity [1], superoxidase activity [1] as well as it is in- here uses immunoaffinity chromatography to volved in Cu transport and homeostasis [1]. Hellman and remove six of