原创力文档chemical profiling and quantification of isoflavone phytoestrogens in kudzu using lcuvmsd异黄酮的化学分析和量化植物雌激素在使用lcuvmsd野葛.pdfVIP
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chemical profiling and quantification of isoflavone phytoestrogens in kudzu using lcuvmsd异黄酮的化学分析和量化植物雌激素在使用lcuvmsd野葛
American Journal of Analytical Chemistry , 2011, 2, 665-674
doi:10.4236/ajac.2011.26076 Published Online October 2011 (http://www.SciRP.org/journal/ajac)
Chemical Profiling and Quantification of Isoflavone
Phytoestrogens in Kudzu Using LC/UV/MSD
1* 2 1
Qing-Li Wu , Yong-Hong Yang , Jim Simon
1
New Use Agriculture and Natural Plant Products Program Department of Plant Biology and Pathology,
Rutgers University, New Brunswick, USA
2Key Laboratory, Department of Plant Pathology, Yunnan Agricultural University, Kunmin, China
*
E-mail : qlwu@
Received February 21, 2011; revised May 3, 2011; accepted May 20, 2011
Abstract
Method development for determination of isoflavones in kudzu was achieved by HPLC/UV/ESI-MSD. Us-
ing three kudzu species of Pueraria lobata, P. thomsonii and P. edulis, and analyzing the isoflavones sepa-
rately by species and from different plant tissues (roots, stems, leaves, flowers and fruits) in each species, a
total of 25 isoflavones were identified by their molecular ions and characteristic fragment ion peaks using
LC/MSD under MS and MS/MS mode, and in comparison with standard isoflavones. Two main chemical
groups were identified: 1) 8-C-glycosyl isoflavone of puerarin and the analogues of 5-OH puerarin, 3’-OH
puerarin, 3’-OMe puerarin, and their glycosides; and 2) daidzein, genistein, glycitein and their glycosyl and
malonyl derivatives, which are similar to those known in soy. To accurately quantitate total isoflavones,
acidic hydrolysis during extraction of kudzu samples was applied to convert the oxygen glycosides i
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