瘢痕患者免疫功能状态探究.doc

瘢痕患者免疫功能状态探究[摘要]目的：通过比较不同程度瘢痕患者和健康对照组的细胞免疫和体液免疫的差异，分析瘢痕患者免疫功能状态，探讨瘢痕可能的免疫学发生机制。方法：101例瘢痕患者分为三组：轻度瘢痕组、中度瘢痕组和重度瘢痕组。选择健康体检者50例做为正常对照组。测定T细胞亚群比例和CD8T淋巴细胞的杀伤活性，同时，检测CD8T淋巴细胞培养上清液中TNF-β水平。分别采用生物学活性法和ELISA测定瘢痕患者和正常对照IFN-γ和IL-4分泌水平。结果：不同组别CD8T细胞亚群比例比较结果和CD4T细胞相似，即在对照组和轻度瘢痕组，中度瘢痕组和重度瘢痕组差异不具有显著性，其余各组间差异具有显著性。血清中IFN-γ在各组间差异均具有显著性; 上述各组血清中IL-4表达水平除在轻度和中度瘢痕组间差异不具有显著性外，其它各组间差异均具有统计学意义。CD8T淋巴细胞杀伤效应在效靶比为5:1时，除轻度瘢痕和中度瘢痕组间差异不具有显著性外，其余各组间差异均具有统计学意义。CD8T细胞培养上清中TNF-β水平在各组间差异均具有显著性。结论：同对照组比较，瘢痕患者适应性免疫出现以细胞免疫功能升高，伴随体液免疫功能降低的趋势，并且随着瘢痕程度的加重，这种变化渐趋明显。提示，瘢痕组织的形成可能与机体细胞免疫功能异常升高和体液免疫功能水平降低有关。 [关键词]瘢痕；Th1型细胞因子；Th2型细胞因子；CD8T细胞 [中图分类号]R619+.6R320.2 [文献标识码]A [文章编号]1008-6455（2011）02-0247-05 Study on the adoptive immunity of patients with scar YAN Gui-chun,AN Xin,ZHANG Hai-yong,WANG Rui-hai,PEI Yin-hui,YU Bo (Department ofPlastic Surgery,Kailuan Hospital,Tangshan 063000,Hebei,China) Abstract:ObjectiveBy comparing the differencesof cellular and humoral immunity betweencaseandcontrol group,the immunity of patients with scar was assessed,so thepossible immunity mechanism of the formation scar was presumed.Methods101 cases of patients with scar were divided into 3 groups (mild, middle and heavy group）,50 ones who have physical examined were served ascontrol. T lymphocytesubtypes and cyotoxicity of CD8 T lymphocyte were detected,and the concentration of TNF-β secreted by CD8 Tlymphocyte was determined too. Serum was collected fo the detections of INF-r and IL-4,bio-activity method and ELISA were performed for that purposerespectively. ResultsThe differences of the percentage of CD4 T subtype in mild,middle,heavy scar group and control were significant except the comparisons between controland mild scar group,middle scar group and heavy scar group, so didthat in CD8 T subtype. The concentraion of INF-r was significantly different among all the groups. The expressions of IL-4 were different except the comparion ofmild andmiddle scar group. About the cytotoxicity of CD8 T lymphocyte,thediff