comparative analysis of micrornas between sporophyte and gametophyte of porphyra yezoensis比较分析小分子核糖核酸的孢子体和配子体之间porphyra yezoensis.pdfVIP

Hindawi Publishing Corporation Comparative and Functional Genomics Volume 2012, Article ID 912843, 9 pages doi:10.1155/2012/912843 Research Article Comparative Analysis of MicroRNAs between Sporophyte and Gametophyte of Porphyra yezoensis Linwen He,1, 2 Aiyou Huang,1, 2 Songdong Shen,3 Jianfeng Niu,1 and Guangce Wang1 1 Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences (IOCAS), Nanhai Road 7, Qingdao 266071, China 2 School of Earth Science, Graduate University of Chinese Academy of Sciences, Yuquan Road 19, Beijing 100049, China 3 College of Life Sciences, Soochow University, Renai Road 199, Suzhou 215123, China Correspondence should be addressed to Guangce Wang, gcwang@ Received 29 March 2012; Revised 6 August 2012; Accepted 22 August 2012 Academic Editor: Paul Denny Copyright © 2012 Linwen He et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Porphyra yezoensis Ueda is an intertidal marine red algae that has received increasing attention as a model organism owing to its important role in biological research and the agronomic industry. The two generations of Porphyra yezoensis, the sporophyte and the gametophyte, have the same genome but show great differences in many aspects, including structural features, habitat, and gene expression. To identify miRNAs and their probable roles in P. yezoensis development, we constructed and sequenced libraries of small RNA from P. yezoensis sporophytes and gametophytes. The sequencing data were analyzed, and 14 miRNAs were identified, with only one common to these two samples. Our results show that P. yezoensis has a