comparative analysis of protein expression concomitant with dna methyltransferase 3a depletion in a melanoma cell line比较分析蛋白表达和dna甲基转移酶3黑素瘤细胞系的损耗.pdfVIP

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comparative analysis of protein expression concomitant with dna methyltransferase 3a depletion in a melanoma cell line比较分析蛋白表达和dna甲基转移酶3黑素瘤细胞系的损耗.pdf

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comparative analysis of protein expression concomitant with dna methyltransferase 3a depletion in a melanoma cell line比较分析蛋白表达和dna甲基转移酶3黑素瘤细胞系的损耗

American Journal of Analytical Chemistry , 2011, 2, 539-572 doi:10.4236/ajac.2011.25064 Published Online September 2011 (http://www.SciRP.org/journal/ajac) Comparative Analysis of Protein Expression Concomitant with DNA Methyltransferase 3A Depletion in a Melanoma Cell Line 1,# 1,# 1* 2 1 Shengnan Tang , Xiaoyan Liu , Tonghua Li , Haoyue Wang , Jiangming Sun , Qian Qiao1 3 2 , Jun Yao , Jian Fei 1 Department of Chemistry, Tongji University, Shanghai, China 2School of Life Science Technology, Tongji University, Shanghai, China 3School of Medicine, Fudan University, Shanghai, China * E-mail : lith@ Received March 17, 2011; revised May 3, 2011; accepted June 1, 2011 Abstract DNA methyltransferase 3A (Dnmt3a), a de novo methyltransferase, has attracted a great deal of attention for its important role played in tumorigenesis. We have previously demonstrated that melanoma is unable to grow in-vivo in conditions of Dnmt3a depletion in a mouse model. In this study, we cultured the Dnmt3a depletion B16 melanoma (Dnmt3a-D) cell line to conduct a comparative analysis of protein expression con-comitant with Dnmt3a depletion in a melanoma cell line. After two-dimensional separation, by gel elec- tro-phoresis and liquid chromatography, combined with mass spectrometry analysis (1DE-LC-MS/M