cucurbitacin b causes increased radiation sensitivity of human breast cancer cells via g2m cell cycle arrestcucurbitacin b导致增加人类乳腺癌细胞的辐射敏感性通过g2m细胞周期阻滞.pdfVIP

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cucurbitacin b causes increased radiation sensitivity of human breast cancer cells via g2m cell cycle arrestcucurbitacin b导致增加人类乳腺癌细胞的辐射敏感性通过g2m细胞周期阻滞.pdf

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cucurbitacin b causes increased radiation sensitivity of human breast cancer cells via g2m cell cycle arrestcucurbitacin b导致增加人类乳腺癌细胞的辐射敏感性通过g2m细胞周期阻滞

Hindawi Publishing Corporation Journal of Oncology Volume 2012, Article ID 601682, 8 pages doi:10.1155/2012/601682 Research Article Cucurbitacin B Causes Increased Radiation Sensitivity of Human Breast Cancer Cells via G2/M Cell Cycle Arrest Suwit Duangmano,1, 2 Phorntip Sae-lim,3 Apichart Suksamrarn,3 Pimpicha Patmasiriwat,1 and Frederick E. Domann2 1 Faculty of Medical Technology, Mahidol University, Bangkok, Thailand 2 Free Radical and Radiation Biology Program, Department of Radiation Oncology, University of Iowa, Iowa City, IA 52242, USA 3 Faculty of Science, Ramkhamhaeng University, Bangkok, Thailand Correspondence should be addressed to Frederick E. Domann, frederick-domann@ Received 23 January 2012; Accepted 27 March 2012 Academic Editor: Julian J. Raﬀoul Copyright © 2012 Suwit Duangmano et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Purpose. To explore the eﬀects of cucurbitacin B on the radiation survival of human breast cancer cells and to elucidate the cellular mechanism of radiosensitization if any. Materials and Methods. Human breast carcinoma cell lines were treated with cucurbitacin B before irradiation with 0–10 Gy of 137Cs gamma rays. The eﬀect of cucurbitacin B on cell-survival following irradiation was evaluated by colony-forming assay. Cell cycle distributions were investigated using ﬂow cytometry. Real-time PCR and western blots were performed to investigate the expression of cell cycle checkpoints. Results. Cucurbitacin B inhibited breast cancer cell proliferation in a dose-dependent manner. Only MDA-MB-231 and MCF7:5C cells but not SKBR-3 cells were radiosensitize