cultured human airway epithelial cells (calu-3) a model of human respiratory function, structure, and inflammatory responses人工培养的气道上皮细胞(calu-3)人类呼吸功能的一个模型,结构,和炎症反应.pdfVIP

Hindawi Publishing Corporation Critical Care Research and Practice Volume 2010, Article ID 394578, 8 pages doi:10.1155/2010/394578 Review Article Cultured Human Airway Epithelial Cells (Calu-3): A Model of Human Respiratory Function, Structure, and Inflammatory Responses Yan Zhu,1 Aaron Chidekel,1, 2, 3 and Thomas H. Shaffer1, 2, 4 1 Nemours Biomedical Research, Nemours Research Lung Center, Alfred I. duPont Hospital for Children, 1600 Rockland Road, Wilmington, DE 19803, USA 2 Department of Pediatrics, Jeff erson Medical College, Thomas Jeff erson University, 1025 Walnut Street, Suite 700, Philadelphia, PA 19107, USA 3 Department of Pediatrics, Alfred I. duPont Hospital for Children, 1600 Rockland Road, Wilmington, DE 19803, USA 4 Departments of Physiology and Pediatrics, School of Medicine, Temple University, 3420 North Broad Street, Philadelphia, PA 19140, USA Correspondence should be addressed to Aaron Chidekel, achidek@ Received 31 January 2010; Revised 16 April 2010; Accepted 3 May 2010 Academic Editor: Hector R. Wong Copyright © 2010 Yan Zhu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This article reviews the application of the human airway Calu-3 cell line as a respiratory model for studying the effects of gas concentrations, exposure time, biophysical stress, and biological agents on human airway epithelial cells. Calu-3 cells are grown to confluence at an air-liquid interface on permeable supports. To model human respiratory conditions and treatment modalities, monolayers are placed in an environmental chamber, and exposed to specific levels of oxygen or other therapeutic moda