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Archaea 2, 31–38 © 2006 Heron Publishing—Victoria, Canada Cultivation of methanogens from shallow marine sediments at Hydrate Ridge, Oregon 1,2,3 1 MELISSA M. KENDALL and DAVID R. BOONE 1 Biology Department, Portland State University, P.O. Box 751, Portland, Oregon 97207–0751, USA 2 Corresponding author (melissa.kendall@) 3 Current address: Department of Microbiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390–9048, USA Received August 22, 2005; accepted February 20, 2006; published online March 10, 2006 Summary Little is known about the methanogenic degrada- known about the types of methanogens that produce methane tion of acetate, the fate of molecular hydrogen and formate or there. the ability of methanogens to grow and produce methane in Generally, methanogenesis is limited in marine sediments cold, anoxic marine sediments. The microbes that produce when sulfate is present. Because of their higher affinity for hy- methane were examined in permanently cold, anoxic marine drogen and acetate, sulfate-reducing bacteria (SRB) out-com- sediments at Hydrate Ridge (44°35′ N, 125°10′ W, depth pete methanogens for these substrates (Oremland and Taylor 800 m). Sediment samples (15 to 35 cm deep) were collected 1978, Holmer and Kristensen 1994). However studies of deep from areas of active methane ebullition or areas where methane ocean sediments and sediments at the Hydrate Ridge have hydrates occurred. The samples were diluted into enrichment demonstrated that methanogenesis does occur in zones with medium with formate, acetate or trimethylamine as catabolic high sulfate concentrations as