decreased anti-inflammatory responses to vitamin d in neonatal neutrophils降低抗炎反应新生儿中性粒细胞的维生素d.pdfVIP

Hindawi Publishing Corporation Mediators of Inflammation Volume 2011, Article ID 598345, 7 pages doi:10.1155/2011/598345 Research Article Decreased Anti-Inflammatory Responses to Vitamin D in Neonatal Neutrophils Daniel Hirsch, Faith E. Archer, Meera Joshi-Kale, Anna M. Vetrano, and Barry Weinberger Division of Neonatology, Department of Pediatrics, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, New Brunswick, NJ 08901, USA Correspondence should be addressed to Barry Weinberger, weinbebi@ Received 29 July 2011; Revised 19 September 2011; Accepted 19 September 2011 Academic Editor: Fulvio D’Acquisto Copyright © 2011 Daniel Hirsch et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Neutrophil activity is prolonged in newborns, suggesting decreased exposure and/or responses to immunosuppressive modulators, such as 1,25-hydroxyvitamin D (1,25-vit D ). We hypothesized that 1,25-vit D suppresses neutrophil activation and that 3 3 3 this response is impaired in newborns. Consistent with this, 1,25-vit D3 decreased LPS-induced expression of macrophage inflammatory protein-1β and VEGF in adult, but not neonatal, neutrophils. Expression of vitamin D receptor (VDR) and 25- hydroxyvitamin D3 -1α-hydroxylase was reduced in neonatal, relative to adult neutrophils. Moreover, 1,25-vit D3 induced VDR gene expression in activated adult, but not neonatal, neutrophils. 1,25-vit D3 also suppressed expression of cyclooxygenase-2 and induced expression of 5-