红花注射液对内毒素性急性肝损伤大鼠BaxBcl-2表达及其比值影响.docVIP

红花注射液对内毒素性急性肝损伤大鼠BaxBcl-2表达及其比值影响摘 要:目的:观察红花注射液对内毒素性急性肝损伤大鼠的干预作用及可能的机制。方法:将清洁级雄性SD大鼠80只随机分为模型组、红花注射液干预组(红花组)、还原型谷胱甘肽干预组(TAD组)及空白对照组,模型组时相点设为6h、24h、48h,红花注射液干预组、还原型谷胱甘肽干预组(TAD组)时相点同模型组;每时相点10只动物;空白对照组10只动物。模型组以腹腔注射内毒素脂多糖(LPS)+D-氨基半乳糖胺(D-GalN)诱导建立大鼠急性肝损伤模型;红花组、TAD组于建模前0.5h分别以红花注射液、还原型谷胱甘肽尾静脉注射;模型组、红花组、TAD组分别于建模后6h、24h、48h及空白对照组于6h时取腹腔静脉血和肝组织,检测血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST),苏木素-伊红(HE)染色观察肝组织病理学变化,免疫组织化学法检测肝组织Bax、Bcl-2蛋白表达并计算其比值(Bax/Bcl-2)。结果:与空白对照组比较,模型组各时间点大鼠血清ALT、AST水平显著升高(均P0.05)。结论:红花注射液对内毒素性急性肝损伤有较好的保护作用,其机制可能与下调肝组织Bax、Bcl-2表达及其比值,抑制肝组织细胞过度凋亡相关。 关键词:红花注射液;内毒素;急性肝损伤;Bax Bcl-2;Bax/Bcl-2 中图分类号:R285.5 文献标识码:A 文章编号:1673-7717(2010)01-0159-04 Effects of Safflower Injectionon on Bax Bcl-2 and Bax/Bcl-2 in Rats with Acute Hepatic Injury CHEN Ying?1,HUANG Xiao?min?2 (1.Zhejiang Chinese Medical University, Hangzhou 310053,Zhejiang,China; 2.Zhejiang Hospital of TCM,Hangzhou 310006,Zhejiang,China) Abstract:Objective:To observe the protective effects and mechanism of safflower injection (SI) on rats with acute hepatic injury (AHI) induced by endotoxin. Methods:80 male Sprague-Dawley rats were randomly divided into ten groups, three SI treated groups, three reduced glutathione treated groups, three AHI model groups, and one control group. The rats were intraperitoneal-injected with Lipopolysaccharide (LPS) plus D-galactosamine (D-GalN) for modeling. The SI treated groups was injected SI into a tail vein, and the reduced glutathione treated groups was was injected reduced glutathione as the positive control. The blood from the abdominal vein and liver tissue samples were obtained at 6h, 24h and 48h after modles established. The contents of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were tested, the hepatic pathological changes were observed with hematoxylin-eosin (HE) stain, and the expression of Bax, Bcl-2 were detected by immunohistochemical studies. Results:Compared with the control group, serum levels of ALT and AST in rats of the