development and application of time-resolved surface plasmon resonance spectrometer时间分辨表面等离子体共振谱仪的发展和应用.pdfVIP

American Journal of Analytical Chemistry , 2011, 2, 589-604 doi:10.4236/ajac.2011.25067 Published Online September 2011 (http://www.SciRP.org/journal/ajac) Development and Application of Time-Resolved Surface Plasmon Resonance Spectrometer * Yan Mao, Yu Bao, Wei Wang, Zhenggang Li, Fenghua Li, Li Niu Changchun Institute of Applied Chemistry, Changchun, China E-mail : lniu@ Received March 15, 2011; revised May 3, 2011; accepted May 16, 2011 Abstract Surface plasmon resonance (SPR) sensor, an optical sensor exploiting special electromagnetic waves-surface plasmon polaritons to probe interactions between an analyte in solution and a molecular recognition element immobilized on the SPR sensor surface, has been widely used in various realms, such as investigating bio- molecular interactions and binding properties, detection of biological and chemical analytes, environmental monitoring, food safety and medical diagnostics. This paper reviews the development of SPR sensors and SPR commercial instruments, and emphatically introduces the time-resolved surface plasmon resonance (TR-SPR) techniques. The excellent performances of high sensitivity and rapid detection are easily achieved with TR-SPR spectrometer, whereas the traditional SPR spectrometer cannot be accomplished. Therefore, TR-SPR spectrometer is appropriate for real time analysis of bio-recognition events and small molecular dy- namics. However, only two commercial TR-SPR instruments have been exhibited at present, Thermo FT-SPR 100 and Autolab ESPRIT (SPRINGLE). Unfortunately, the high-priced instruments are not suitable for extensive ap