原创力文档differential proteome analysis of chlamydomonas reinhardtii response to arsenic exposure差异蛋白质组分析,衣藻reinhardtii应对砷暴露.pdfVIP
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differential proteome analysis of chlamydomonas reinhardtii response to arsenic exposure差异蛋白质组分析,衣藻reinhardtii应对砷暴露
American Journal of Plant Sciences, 2012, 3, 764-772
/10.4236/ajps.2012.36092 Published Online June 2012 (http://www.SciRP.org/journal/ajps)
Differential Proteome Analysis of Chlamydomonas
reinhardtii Response to Arsenic Exposure
1 2* 3 1
Chamari Walliwalagedara , Harry van Keulen , Belinda Willard , Robert Wei
1Department of Chemistry, Cleveland State University, Cleveland, USA; 2Department of Biological, Geological, and Environmental
Sciences, Cleveland State University, Cleveland, USA; 3Lerner Research Institute, Cleveland Clinic, Cleveland, USA.
Email: h.vankeulen@
Received February 28th, 2012; revised April 5th, 2012; accepted April 16th, 2012
ABSTRACT
The fresh water unicellular green alga Chlamydomonas reinhardtii was used to explore whether it could function as a
model system to identify proteins that are differentially expressed in response to arsenate exposure. Cells were treated
with different concentrations of arsenate ranging from 100 - 400 M. When exposed to 200 M arsenate, the amount of
live cells started to lessen on the second day and continued to diminish, indicating a toxic effect of arsenate. Proteomic
analysis was used to investigate if these cells showed a specific response to arsenic-induced stress. Fifteen proteins were
found that were over-expressed in the 200 M arsenate-treated samples and two proteins were found to be very strongly
over-expressed in samples treated with 400 µM. These were selected for identification using liquid chromatography
coupled with tandem mass spectrometry. Oxidative stress and protein damage were the major effects as shown by the
up-regulation of Mn-superoxide dismutase, an oxygen-evolving enhancer protein, a chaperonin-like protein and a heat
shock protein.
Keywords: Arsenic Stress; Chlamydomonas reinhardtii; Heavy Metal; Oxygen Stres
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