differential response to moderate uv-b irradiation of two heterocystous cyanobacteria isolated from a temperate ricefield微分反应温和的uv - b辐射的两个heterocystous蓝藻隔绝ricefield温和.pdfVIP

Advances in Microbiology, 2012, 2, 37-47 /10.4236/aim.2012.21006 Published Online March 2012 (http://www.SciRP.org/journal/aim) Differential Response to Moderate UV-B Irradiation of Two Heterocystous Cyanobacteria Isolated from a Temperate Ricefield Germán Pérez, Soledad Doldán, Omar Borsani, Pilar Irisarri Departamento Biología Vegetal, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay Email: irisarri@.uy Received December 15, 2011; revised January 4, 2012; accepted January 15, 2012 ABSTRACT In cyanobacteria both photosynthesis and nitrogen fixation can be affected by UV radiation. Two of the most abundant heterocystous cyanobacteria isolates from a temperate ricefield in Uruguay belonging to Anabaena and Calothrix genus were exposed for 1 or 3 hours to UV-B dosis similar to those to which they are exposed in summer. Anabaena survival after 1 h of UV-B exposure was 10% whereas in Calothrix’s was 30%. Both the quantum yields of photosybtem II fluorescence and O2 photoevolution decreased with time of UV-B exposure for Calothrix and only till 1 h for Anabaena . Only the Calothrix strain presented phycoerithryn as antenna pigment and constitutive UV-B screening mycosporine like aminoacids. In the Anabaena strain, nitrogenase activity was drastically reduced with UV-B irradiation but in Ca- lothrix was not affected. Proline content and lipid peroxidation increased after 3 hours of UV-B exposure only in Ana- baena sp. The antioxidant enzyme activities evaluated followed different trends for both isolates, with an increase in superoxide dismutase activity in the Calothrix isolate. These results show that the two nitrogen-fixing cyanobacteria studied have different responses to UV-B radiation and that cyanobacteria diversity ma