development of t lymphocytes in the nasal-associated lymphoid tissue (nalt) from growing wistar rats发展nasal-associated t淋巴细胞的淋巴组织(nalt)增长wistar鼠.pdfVIP

Clinical Developmental Immunology, March 2004, Vol. 11 (1), pp. 29–34 Development of T Lymphocytes in the Nasal-associated Lymphoid Tissue (NALT) from Growing Wistar Rats ´ GUSTAVO A. SOSA and MARIA E. ROUX* Laboratory of Cellular Immunology, CONICET, Faculty of Pharmacy and Biochemistry, Department of Biological Sciences, University of Buenos Aires, ´ Junın 956 5th floor, C1113AAD, Buenos Aires, Argentina The aim of the present report was to study the development of several T-lymphocyte subsets in the nasal-associated lymphoid tissue (NALT) of growing Wistar rats. CD5þ and CD4þ lymphocytes gradually increased with age. A predominance of CD8aþ over CD4þ T cells was found from 7 to 45 days but from 45 to 60 days of age T helper cells outnumbered the cytotoxic subpopulation. The majority of CD8þ T lymphocytes expressed the heterodimeric isoform. The most relevant findings by immunohistochemistry are: (1) the predominance of TCRgdþ and CD8aþ cells at 7 days postpartum over all the other T-cell subpopulations; and (2) that TCRgdþ outnumbered TCRabþ T cells from 7 to 45 days postpartum whereas ab T cells predominated in 45- and 60-day-old rats. Besides, cytometric studies have shown that the percentages of TCRgdþ , CD8aþ , as well as the population coexpressing both phenotypes (TCRgdþCD8aþ), were significantly higher in rats at 7 days postpartum when compared to 60 day-old rats. In the present study, the finding of a high number of gdþ an