肝素寡糖对人外周血T细胞分泌白介素-4及白介素-5影响.docVIP

肝素寡糖对人外周血T细胞分泌白介素-4及白介素-5影响摘要：目的研究肝素寡糖（Oligs）对人外周血T细胞（PBTLs）分泌白介素-4（IL-4）和白介素-5（IL-5）的抑制作用。方法用3种方法降解肝素并用凝胶过滤色谱法制备肝素寡糖。抽取10例过敏性嗜酸粒细胞性胃肠炎患者的PBTLs用植物凝集素（PHA）和Oligs处理，用ELISA法测定PBTLs培养的上清液分泌IL-4和IL-5的含量。 结果Oligs样品浓度5 μg/mL时，可显著抑制PBTLs对细胞因子IL-4、IL-5的分泌。但是，不同相对分子质量（Mr）的Oligs对IL-4和IL-5具有不同的作用。对IL-4抑制作用最强的是用过氧化氢降解肝素所得Mr为1 142的四糖。抑制IL-5作用最强的是用β-消除法降解肝素所得Mr为1 806的六糖。结论Oligs对人PBTLs分泌IL-4和IL-5的抑制作用与分子结构密切相关，并且有一个基本结构。对IL-4和IL-5抑制作用最强的分别是四糖和六糖。 关键词：肝素寡糖；人外周血T细胞；白介素-4；白介素-5 中图分类号：TQ464文献标识码：A文章编号：1672-979X(2008)11-0001-05 Effect of Heparin-Derived Oligosaccharides on Secretion of Interleukin-4 and Interleukin-5 from Human Peripheral Blood T Lymphocytes JI Sheng-li1, ZHANG Yun-feng1, CUI Hui-fei1, SHI Feng1, CHI Yan-qing2, CAO Ji-chao1 (1. Institute of Biochemical and Biotechnological Drug, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China; 2. Department of Pharmacy, Shandong Provincial Hospital, Jinan 250021, China) Abstract：Objective To study the inhibitory effect of heparin-derived oligosaccharides (Oligs) on the secretion of IL-4 and IL-5 from human peripheral blood T lymphocytes (PBTLs). Methods Heparin was degraded by three different methods and low molecular weight heparins (LMWHs) were purified with gel-filtration chromatography in order to prepare Oligs. The PBTLs from ten patients with allergic eosinophilic gastroenteritis were treated with phytohemagglutinin (PHA) and Oligs, and the concentrations of IL-4 and IL-5 in the cell culture supernatant fluid were determined by ELISA. Results 5 μg/mL of Oligs samples could obviously inhibit the secretion of IL-4 and IL-5 from human PBTLs, while Oligs with different molecular weights（Mr）had different effects on the secretion of IL-4 and IL-5. A tetrasaccharide with Mr of 1 142 obtained by H2O2 degradation could most inhibit the secretion of IL-4, while a hexasaccharide with Mr of 1 806 obtained by beta-elimination could inhibit the secretion of IL-5 most. Conclusion The inhibitory effec