distribution of nuclei of different ploidy levels during ovule, seed and protocorm development in phalaenopsis aphrodite subsp. formosana (orchidaceae)核的分布不同倍性水平在胚珠,种子和原球茎蝴蝶兰属阿佛洛狄忒无性系种群的发展。.pdfVIP
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distribution of nuclei of different ploidy levels during ovule, seed and protocorm development in phalaenopsis aphrodite subsp. formosana (orchidaceae)核的分布不同倍性水平在胚珠,种子和原球茎蝴蝶兰属阿佛洛狄忒无性系种群的发展。
American Journal of Plant Sciences , 2011, 2, 325-333 325
doi:10.4236/ajps.2011.23037 Published Online September 2011 (http://www.SciRP.org/journal/ajps)
Distribution of Nuclei of Different Ploidy Levels
during Ovule, Seed and Protocorm Development
in Phalaenopsis aphrodite subsp. formosana
(Orchidaceae)
1 1 1 2
Goamg-Tyng Jean , Yu-Lin Kao , Ching-Yan Tang , Wen-Huei Chen
1Department of Life Sciences Institute of Biotechnology, National University of Kaohsiung, Kaohsiung, Taiwan, Chinese Taipei;
2Orchid Research Center, National Cheng Kung University, Tainan, Taiwan, Chinese Taipei.
Email: wenhueic005@, yulinkao@.tw
Received April 6th, 2011; revised May 8th, 2011; accepted June 2nd, 2011.
ABSTRACT
Distribution of nuclei of different ploidy levels was studied at different developmental stages in the embryonic tissue of
the ovule, seed and protocorm of Phalaenopsis aphrodite subsp. formosana (Miwa) E.A. Christ by a combination of
flow cytometry and fluorescence microscopy with Apo Tome Slider. Three stages of ploidy patterns were identified in
the ovular tissue at different days after pollination (DAP). Firstly, between pollination and fertilization (0 to 50 DAP),
2C nuclei were dominant over 4C nuclei and resulted in low level of cycle value. Secondly, between fertilization and
seed maturation (50 to 110 DAP), amount of 4C nuclei increased rapidly, maintained at a high level and then de-
creased gradually to a low level. Small amount of 8C nuclei was also detected at this stage. Thirdly, at seed maturation
(110 to 130 DAP), 2C nuclei became dominant over 4C nuclei again and the cycle value remained at a low but signifi-
cant level at this stage. After seed sowing, nuclei with ploidy levels of 2C, 4C and 8C were obs
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