effect of assay conditions on the measurement of dehydrogenase activity of streptomyces venezuelae using triphenyl tetrazolium chloride试验条件对测量的影响的脱氢酶活性链霉菌属venezuelae使用氯化三苯四唑.pdfVIP

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effect of assay conditions on the measurement of dehydrogenase activity of streptomyces venezuelae using triphenyl tetrazolium chloride试验条件对测量的影响的脱氢酶活性链霉菌属venezuelae使用氯化三苯四唑.pdf

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effect of assay conditions on the measurement of dehydrogenase activity of streptomyces venezuelae using triphenyl tetrazolium chloride试验条件对测量的影响的脱氢酶活性链霉菌属venezuelae使用氯化三苯四唑

Advances in Bioscience and Biotechnology, 2011, 2, 214-225 ABB doi:10.4236/abb.2011.24032 Published Online August 2011 (http://www.SciRP.org/journal/abb/). Effect of assay conditions on the measurement of dehydrogenase activity of Streptomyces venezuelae using triphenyl tetrazolium chloride * Tracey Burdock, Marianne Brooks , Abdel Ghaly, Deepika Dave Department of Process Engineering and Applied Science, Dalhousie University, Halifax, Canada. * Email: Su-Ling.Brooks@dal.ca Received 9 April 2011; revised 19 June 2011; accepted 20 July 2011. ABSTRACT 1. INTRODUCTION Jadomycins are type II polyketide synthesis-derived Jadomycin is an antibiotic that has shown activities secondary metabolites produced by the actinomycete against bacteria, yeasts and fungi as well as cytotoxic properties to cancer cells. Because of the wide range Streptomyces venezuelae [1]. Jadomycins have demon- of its inhibitory actions, jadomycin shows promise as strated antibacterial, antitumor, antifungal and enzyme a novel antibiotic and cancer treatment drug. Strep- inhibitory functions as well as cytotoxic properties to the tomyces venezuelae are aerobic bacteria that produce cancer cells [2]. They are considered to be promising jadomycin and the size of bacterial population can novel antibiotics and cancer treatment drugs [3]. The pro- significantly affect the yield of jadomycin. Therefore, duction of jadomycin takes place in a nu