effect of calcium and diltiazem on phenylhydrazine-induced oxidative injury in goat erythrocytes钙和地尔硫卓对phenylhydrazine-induced羊红细胞氧化损伤.pdfVIP

Vol.2, No.10, 1221-1225 (2010) Health doi:10.4236/health.2010.210181 Effect of calcium and diltiazem on phenylhydrazine-induced oxidative injury in goat erythrocytes Kaushik Das*, Jharna Bhattacharyya Division of Cell Biology and Physiology, Indian Institute of Chemical Biology, Kolkata, India; *Corresponding Author: kaushik.17@ Received 8 June 2010; revised 22 June 2010; accepted 7 July 2010. ABSTRACT cell-defence system, altering its homeostatic capacity. PHZ, in presence of haemoglobin autooxidizes to form Lipid peroxidation, hemolysis and thiol contents hydrogen peroxide, which leads to hemolysis, resulting were studied in intact goat erythrocytes exposed in severe haemolytic anaemia [2] and generates reactive to phenylhydrazine. An increase in lipid peroxi- oxygen species [3] among which hydroxyl radical, OH· [4] dation, hemolysis and thiol content was observ- is highly reactive and initiates the peroxidation of unsa- ed after phenylhydrazine treatment of erythrocy- 2+ turated fatty acids in endogenous phospholipids. Oxida- te. Extracellular Ca potentiates the phenylhy- tive stress due to overproduction of reactive species pl- drazine-induced lipid peroxidation and hemoly- 2+ ays a signific