原创力文档effect of caralluma fimbriata extract on 3t3-l1 pre-adipocyte cell division影响caralluma fimbriata提取3 t3-l1 pre-adipocyte细胞分裂.pdfVIP
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effect of caralluma fimbriata extract on 3t3-l1 pre-adipocyte cell division影响caralluma fimbriata提取3 t3-l1 pre-adipocyte细胞分裂
Food and Nutrition Sciences, 2011, 2, 329-336
doi:10.4236/fns.2011.24047 Published Online June 2011 (/journal/fns)
Effect of Caralluma Fimbriata Extract on 3T3-L1
Pre-Adipocyte Cell Division
1 2 3 4
Soundararajan Kamalakkannan , Ramaswamy Rajendran , Ramasamy V. Venkatesh , Paul Clayton ,
Mohammad A. Akbarsha5,6
1Department of Animal Science, Bharathidasan University, Tiruchirappalli, India; 2GreenChem, Bangalore, India; 3Gencor Pacific Limited,
Discovery Bay, Hong Kong (China); 4Department of Life Sciences, Oxford Brookes University, Oxford, UK; 5Mahatma Gandhi-Doerenk-
amp Center for Alternatives, Bharathidasan University, Tiruchirappalli, India; 6College of Food Science and Agriculture, King Saud Univer-
sity, Riyadh, Kingdom of Saudi Arabia.
Email: akbarbdu@
rd th st
Received July 3 , 2010; revised August 18 , 2010; accepted September 21 , 2010.
ABSTRACT
A standardized extract of the plant Caralluma fimbriata (Slimaluma™) is widely used in the management of obesity but
its mode of action is not yet clarified. This study investigated the ability of Caralluma fimbriata extract (CFE) to modify
pre-adipocyte cell division and thus the development of hyper-plastic obesity . Mouse 3T3-L 1 pre-adipocyte cell line
samples were treated with different concentrations of an extract of CFE standardized against its pregnane glycoside
content. Plain medium formed the negative control and hydroxyurea was the positive control. The cells were counted at
12-hour intervals, and their viability tested using the MTT assay. The treated cells were subjected to direct and indirect
immunofluorescent assays for cyclin D 1. CFE inhibited 3T3-L 1 cell growth in a dose and duration-dependent manner,
with results comparable
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