effect of opium on lipid profile and expression of liver x receptor alpha (lxrα) in normolipidemic mouse鸦片对血脂的影响和表达肝x受体α(lxrα)normolipidemic鼠标.pdfVIP

Food and Nutrition Sciences, 2012, 3, 249-254 /10.4236/fns.2012.32036 Published Online February 2012 (http://www.SciRP.org/journal/fns) Effect of Opium on Lipid Profile and Expression of Liver X Receptor Alpha (LXRα) in Normolipidemic Mouse 1,2 3* 4 3 Abbas Mohammadi , Ebrahim Abbasi Oshaghi , Arash Noori Sorkhani , Farhad Oubari , Roghaye Hosseini Kia3, Ali Rezaei5 1Department of Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran; 2Physiology Research Centre, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran; 3Department of Laboratory Science, Islamic Azad University, Borūjerd Branch, Borūjerd, Iran; 4Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran; 5Department of Basic Science, Tehran Payame Noor University, Tehran, Iran. * Email: 7abbasi@ Received November 2nd, 2011; revised December 19th, 2011; accepted December 27th, 2011 ABSTRACT Many people believe that opium has beneficial effects on lipid profile which results in reduced atherosclerosis. Opium contains several alkaloids and biological active components, which some of them are used for atherosclerosis treatment. The liver X receptor α (LXRα) is an important regulator of cholesterol and glucose homeostasis that be- longs to the nuclear receptor superfamily. This study aimed to investigate the effects of opium on glucose, lipid pro- file and LXRα expression. Sixteen N-mary mice randomly were divided into two groups (control and addict), and were studied for one month. Serum lipid profile, Fasting blood sugar (FBS), Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) were determined. Also LXR mRNA and protein levels were determined by Reverse Transcription PCR and western blot