effect of oxygen and redox potential on glucose fermentation in thermotoga maritima under controlled physicochemical conditions氧气和氧化还原电位对葡萄糖发酵thermotoga maritima控制物理化学条件.pdfVIP

Hindawi Publishing Corporation International Journal of Microbiology Volume 2010, Article ID 896510, 10 pages doi:10.1155/2010/896510 Research Article Effect of Oxygen and Redox Potential on Glucose Fermentation in Thermotoga maritima under Controlled Physicochemical Conditions Raja Lakhal,1 Richard Auria,1 Sylvain Davidson,1 Bernard Ollivier,1 Alain Dolla,2 Moktar Hamdi,3 and Yannick Combet-Blanc1 1 UMR D180, IRD, ESIL, Universit´es de Provence et de la M´editerran´ee, 163 Avenue de Luminy, Case 925, 13288 Marseille Cedex 09, France 2 Unit´e Interactions et Modulateurs de R´eponses, IFR88, CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France 3 Laboratoire d’Ecologie et de Technologie Microbienne, Institut National des Sciences Appliqu´ees et de Technologies (INSAT), Universit´e 7 Novembre Carthage, 2 Boulevard de la Terre, BP 676, 1080 Tunis, Tunisia Correspondence should be addressed to Yannick Combet-Blanc, bet-blanc@univmed.fr Received 8 October 2010; Revised 6 December 2010; Accepted 22 December 2010 Academic Editor: Alfons J. M. Stams Copyright © 2010 Raja Lakhal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Batch cultures of Thermotoga maritima were performed in a bioreactor equipped with instruments adapted for experiments performed at 80◦C to mimic the fluctuating oxidative conditions in the hot ecosystems it inhabits. When grown anaerobically on glucose, T. maritima was shown to significantly decrease the redox potential (Eh) of the culture medium down to about −480 mV, as long as glucose was available. Addition of oxygen into T. maritima cult