葡萄籽提取物原花青素对皮肤急性光损伤Fas蛋白及Bcl-2蛋白表达影响.docVIP

葡萄籽提取物原花青素对皮肤急性光损伤Fas蛋白及Bcl-2蛋白表达影响[摘要]目的：评估外用葡萄籽提取物原花青素对急性光损伤的Fas蛋白及Bcl-2蛋白表达的影响。方法：实验设计正常皮肤组、单纯2MED照射组、基质＋2MED照射组、含葡萄籽提取物原花青素样品外用＋2MED照射组共四组，连续3天同样处理24h后对皮肤取材，进行Fas蛋白及Bcl-2蛋白免疫组化染色。结果：含葡萄籽提取物原花青素样品外用＋2MED照射组的Fas蛋白表达积分与单纯2MED照射比较明显下降，Bcl-2蛋白表达积分明显增加，接近正常皮肤。结论：葡萄籽提取物原花青素对日晒伤具有一定的防护作用，其机制可能是与Fas蛋白及Bcl-2蛋白表达相关。 [关键词]葡萄籽提取物原花青素；光损伤；Fas 蛋白, Bcl-2蛋白 [中图分类号]R758.14Q591.2[文献标识码]A[文章编号]1008-6455（2008）03-03 Influence of Grape Seed Proanthocyanidin Extract on protein expression from acute ultraviolet injury YUAN Xiao-ying1,LIU Wei1,XIE Yan-fei,GUO Guang-jin1,MENG Ru-song1,FAN Li-ying1,ZHANG Jie1,TIAN Yan1,MA Hui-min1,XIN Xu-ya1 (1. Department of Dermatology, the Air Force General Hospital of PLA, Beijing 100036,China; 2. Department of Dermatology, the Otorhinolaryngology Hospital of Baitou, Baitou 062510, Hebei,China) Abstract:ObjectiveIn this study, the Influence of Grape Seed Proanthocyanidin Extract on Fas Protein and Bcl-2 protein expression from acute ultraviolet injury was investigated in skin by solar-simulator radiation. MethodsAreas of skin of normal volunteers were divided into four sites: normal untreated skin, skin treated with 2-MED UV radiation, or skin treated with GPSE solutions or Vehicle 30minutes before 2-MED solar simulated radiation. Skin areas of different sites were examined immunohistochemically for Fas Protein and Bcl-2 protein expression.ResultsOn immunohistochemical examination, Skin treated with GSPE before UV radiation reduced Fas protein expression and upregulated Bcl-2 protein expression compared with skin treated only with UV radiation.ConclusionGrape Seed Proanthocyanidin Extract may protect skin against acute sunburn partly through reducing Fas protein expression and upregulated Bcl-2 protein expression. Key words: Grape Seed Proanthocyanidin Extract, sunburn, Fas , Bcl-2 随着生活水平的提高，人们进行越来越多的户外运动、日光浴，导致接受过多的紫外线照射。研究表明皮肤在急性紫外光损伤过程中，大量氧自由基生成、细胞内钙超载、大量炎性细胞浸润以及线粒体损伤等均可促使皮肤DNA损伤，从而导致角质形成细胞调亡。在细胞调亡过程中，调亡相关的蛋白表达产物Fas蛋白及抗凋亡因子Bc